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DeadEnd Fluorometric TUNEL System
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DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System
DeadEnd Fluorometric TUNEL System from Promega
(1)
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Citations:
(645)
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DeadEnd Fluorometric TUNEL System
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Description
DNA fragmentation detection assay. Designed for the detection and quantitation of apoptotic cells within a cell population
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Fluorometric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3250
Quantity
60 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (645)
(1)
Anti-Apoptosis Therapy for Meniscal Avascular Zone Repair: A Proof-of-Concept Study in a Lapine Model
Bioengineering (Basel)
December 14, 2023
Wenqiang Yan, Yue Wu, Fengyuan Zhao, Ruilan Dai, Yunan Zhou, Dingge Liu, Jin Cheng, Xiaoqing Hu, Yingfang Ao
examination was completed according to the manufacture’s protocols (
DeadEnd™ Fluorometric TUNEL System
,
G3250
,
Promega
, USA). Briefly, the slices were permeabilized with 20 ug/mL proteinase K solution, and then
(2)
Epigallocatechin gallate prevents cardiomyocytes from pyroptosis through lncRNA MEG3/TAF15/AIM2 axis in myocardial infarction
Chin Med
December 6, 2023
Chaoshi Qin, Tingting Wang, Ni Qian, Jing Liu, Rong Xi, Qing Zou, Hui Liu, Xiaolin Niu
fragmentation in HL-1 cells were measured by the
DeadEnd™ Fluorometric TUNEL System
(
Promega
, Madison, WI, USA;
G3250
). Cells were stained by DAPI to identify the nuclei followed by visualization under
fluorescence microscope
.
(3)
Cerebral glucagon-like peptide-1 receptor activation alleviates traumatic brain injury by glymphatic system regulation in mice
CNS neuroscience & therapeutics
December 1, 2023
Lv C, Han S, Sha Z, Liu M, Dong S, Zhang C, Li Z, Zhang K, Lu S, Xu Z,
et al
.
software. 2.12
TUNEL
staining
Apoptotic cells were detected by
TUNEL assay
using the
TUNEL assay
kit (
G3250
,
Promega
) according to the manufacturer's instructions. Cortical
neurons
were labeled using
NeuN
immunofluorescence
(4)
Novel phenoxy-((phenylethynyl) selanyl) propan-2-ol derivatives as potential anticancer agents
BMC Chemistry
November 28, 2023
Wenxin Xu, Yali Du, Beibin Pan, Qiying Wang, Haoran Zheng, Ruonan Zhang, Jiaxin Lou, Guanghui Zhu, Jie Zhou, Jian Sun
penicillin/streptomycin were purchased from
Gibco
. The
DeadEnd™ Fluorometric TUNEL System
TB235 kit (
G3250
) was from
Promega
(Southampton, UK), and the
Annexin V-FITC/PI Apoptosis Detection Kit
(
A211-01
,
Vazyme
)
(5)
Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application
Cell Reports Medicine
November 21, 2023
Xiaoxue Ren, Mingle Huang, Weixiang Weng, Yubin Xie, Yifan Wu, Shenghua Zhu, Ying Zhang, Dongming Li, Jiaming Lai, Shunli Shen,
et al.
assays
,Critical commercial
assays
,Critical commercial
assays
,,
DeadEnd™ Fluorometric TUNEL System
,
Promega
,Cat#
G3250
REAL™ EnVision™ Detection System,
Dako
,Cat#
K500711-2
CellTiter-Glo
assay
,
Promega
,Cat#
G7570
PureLink™
(6)
LncRNA AC125982.2 regulates apoptosis of cardiomyocytes through mir-450b-3p/ATG4B axis in a rat model with myocardial infarction
Heliyon
November 17, 2023
Zhi-jun Xu, Peng-yin Zhang, Zhen-qiu Li, Han-ping Zhu, Wei-lu Tan, Pei-hua Ren
of U6. 2.8
TUNEL
staining
Primary
cardiomyocyte
apoptosis assay
was performed using the
TUNEL
kit (
G3250
,
Promega
, USA). The medium for
cell culture
was disposed of and rinsed with PBS once. Permeabilization
(7)
Endogenous FGF1 Deficiency Aggravates Doxorubicin-Induced Hepatotoxicity
Toxics
November 12, 2023
Chunjie Gu, Zijuan Liu, Yingjian Li, Mei Yi, Simeng Wang, Xia Fan, Da Sun, Chi Zhang, Xiaoqing Yan, Guicheng Wu
Labeling
(
TUNEL) Assay
Apoptosis was detected by
TUNEL
staining
(
Promega
, Madison, WI, USA, Cat #
G3250
) per the manufacturer’s instructions. In brief, the deparaffinized and rehydrated liver sections were
(8)
Specific heterozygous variants in MGP lead to endoplasmic reticulum stress and cause spondyloepiphyseal dysplasia
Nature Communications
November 3, 2023
Ophélie Gourgas, Gabrielle Lemire, Alison L. Eaton, Sultanah Alshahrani, Angela L. Duker, Jingjing Li, Ricki S. Carroll, Stuart Mackenzie, Sarah M. Nikkel, Michael B. Care4Rare Canada Consortium,
et al.
on both fixed
HEK-293 cells
and tissue sections using a
DeadEnd Fluorometric TUNEL System
(
Promega
#
G3250
) following the instructions of the manufacturer. Lumbar vertebrae samples from 3-week-old
mice
were
(9)
Patient-derived precision cut tissue slices from primary liver cancer as a potential platform for preclinical drug testing
eBioMedicine
November 1, 2023
Ravi Jagatia, Ewald J. Doornebal, Una Rastovic, Nicola Harris, Moyosoreoluwa Feyide, Anabel Martinez Lyons, Rosa Miquel, Yoh Zen, Ane Zamalloa, Farooq Malik,
et al.
evaluation of apoptosis, tissue sections were stained using Dead-end Fluorometric
TUNEL
Kit (
Promega
,
G3250
). All samples were mounted with
Fluoroshield mounting medium with DAPI
(
Abcam
,
ab104135
). Images were
(10)
A human mitofusin 2 mutation can cause mitophagic cardiomyopathy
eLife
November 1, 2023
Antonietta Franco, Jiajia Li, Daniel P Kelly, Ray E Hershberger, Ali J Marian, Renate M Lewis, Moshi Song, Xiawei Dang, Alina D Schmidt, Mary E Mathyer,
et al.
hematoxylin-eosin (Sigma, GHS116 and HT110116) or
TUNEL
labeled using the manufacturer’s protocol (
Promega
,
G3250
).
Transmission electron microscopy
of
mouse
heart tissues fixed in 4% paraformaldehyde, 2.5% glutaraldehyde
(11)
B7 Induces Apoptosis in Colorectal Cancer Cells by Regulating the Expression of Caspase-3 and Inhibits Autophagy
OncoTargets and Therapy
October 27, 2023
Xinyi Zhang, Fengxi Li, Rong Li, Nan Zhao, Dianfeng Liu, Yuelin Xu, Lei Wang, Dongxu Wang, Ruihong Zhao
stained with terminal deoxynucleotidyl transferase
dUTP
nick end
labeling
(
TUNEL
) reagent (
Promega
,
g3250
) for 20 min. Hoechst 33,342 (10 ng/mL, Thermo science, USA) was used for nuclei visualization. The apoptotic
(12)
A short-term high-fat diet alters rat testicular activity and blood-testis barrier integrity through the SIRT1/NRF2/MAPKs signaling pathways
Front Endocrinol (Lausanne)
October 27, 2023
Sara Falvo, Sergio Minucci, Alessandra Santillo, Rosalba Senese, Gabriella Chieffi Baccari, Massimo Venditti
apoptotic cells were identified in paraffin sections through the
DeadEnd™ Fluorometric TUNEL System
(#
G3250
;
Promega
Corp., Madison, WI, USA) following the manufacturer’s protocol, with little modifications.
(13)
A heterozygous ZP2 mutation causes zona pellucida defects and female infertility in mouse and human
iScience
October 20, 2023
Sai-Li Liu, Hai-Yang Zuo, Bing-Wang Zhao, Jia-Ni Guo, Wen-Bo Liu, Wen-Long Lei, Yuan-Yuan Li, Ying-Chun Ouyang, Yi Hou, Zhi-Ming Han,
et al.
#SMK-101 BCA protein assay kit,Biosharp,Cat #BL521A
DeadEnd™ Fluorometric TUNEL System
,
Promega
,Cat #
G3250
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,, 293T,"Provided
(14)
Regulated adipose tissue-specific expression of human AGPAT2 in lipodystrophic Agpat2-null mice results in regeneration of adipose tissue
iScience
October 20, 2023
Anil K. Agarwal, Katie Tunison, Goncalo Vale, Jeffrey G. McDonald, Xilong Li, Philipp E. Scherer, Jay D. Horton, Abhimanyu Garg
Transcription Reagents kit,
Fisher Scientific
,Cat#
N8080234
DeadEnd™ Fluorometric System,
Promega
,Cat#
G3250
Mouse Leptin ELISA Kit,Crystall Chem,Cat# 90030 TMT10plex Isobaric Mass Tagging Kit,
Thermo Scientific
,Cat#
(15)
Early Resveratrol Treatment Mitigates Joint Degeneration and Dampens Pain in a Mouse Model of Pseudoachondroplasia (PSACH)
Biomolecules
October 20, 2023
Jacqueline T. Hecht, Alka C. Veerisetty, Debabrata Patra, Mohammad G. Hossain, Frankie Chiu, Claire Mobed, Francis H. Gannon, Karen L. Posey
transferase-mediated deoxyuridine triphosphate-biotin nick end
labeling
(
TUNEL
)
staining
using the
Promega
G3250
kit following the manufacturer’s protocol. For proteoglycan stains, samples were deparaffinized, hydrated
(16)
CHEK2 signaling is the key regulator of oocyte survival after chemotherapy
Science advances
October 20, 2023
Emori C, Boucher Z, Bolcun-Filas E.
Medical). Antibody co-immunostaining with
TUNEL
for apoptotic cells (DeadEnd Fluorometric system,
Promega
,
G3250
) was performed by standard
immunofluorescence
protocol followed by
TUNEL
staining
according to the manufacturer’s
(17)
Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury
Cell Death & Disease
October 10, 2023
Wenjia Xie, Qingyun He, Yan Zhang, Xinxin Xu, Ping Wen, Hongdi Cao, Yang Zhou, Jing Luo, Junwei Yang, Lei Jiang
h. The cells were counted by measuring absorbance at 450 nm.
TUNEL
staining
TUNEL
staining
(cat:
G3250
,
Promega
) was performed according to the manufacturer’s instructions. Positive-stained nucleus were
(18)
Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment
Autophagy
October 1, 2023
Li Q, Zhao Y, Guo H, Li Q, Yan C, Li Y, He S, Wang N, Wang Q.
quantitative analysis.
TUNEL assay
To detect neuronal apoptosis in the brain,
TUNEL assay
(
Promega
,
G3250
) was performed on brain sections according to the manufacturer’s instructions. The stained sections
(19)
Supplementation of Medium-Chain Triglycerides Combined with Docosahexaenoic Acid Inhibits Amyloid Beta Protein Deposition by Improving Brain Glucose Metabolism in APP/PS1 Mice
Nutrients
October 1, 2023
Zehao Wang, Dalong Zhang, Cheng Cheng, Zhenzhen Lin, Dezheng Zhou, Yue Sun, Wen Li, Jing Yan, Suhui Luo, Zhiyong Qian,
et al.
30 min, processed with 0.5% Triton-X for 30 min, and then incubated with the rTDT reaction system (
G3250
,
Promega
, Shanghai, China) for 60 min at 37 °C in the dark. The reaction was then terminated using a
(20)
Free Bilirubin Induces Neuro-Inflammation in an Induced Pluripotent Stem Cell-Derived Cortical Organoid Model of Crigler-Najjar Syndrome
Cells
September 14, 2023
Abida Islam Pranty, Wasco Wruck, James Adjaye
1.53c. 2.4.
TUNEL Assay
Apoptotic cells were detected using the
DeadEnd™ Fluorometric TUNEL System
(
Promega
,
G3250
, Madison, WI, USA) following the manufacturer’ s protocol. 2.5.
Reverse Transcriptase PCR
(
RT-PCR
) The
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Reviews
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(1)
8/11/2015
Detecting and Quantifying Apoptotic Cells using DeadEnd Fluorometric TUNEL System
Lenin Veeraval
This kit was used to detect and quantify the apoptotic cells in a cell population treated with anticancer compounds compared to control cells. The product is simple to use and has high throughput.
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DeadEnd Fluorometric TUNEL System from Promega
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