Description
The Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA assay kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a monoclonal antibody directed against a distinct Antigensic determinant on the intact SLPI molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a HRP conjugated anti-SLPI monoclonal antibody. Excess anti-SLPI-HRP antibody is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if SLPI is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of SLPI is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm