Description
CST's PathScan® Phospho-Met (panTyr) Sandwich ELISA Kit #7333 detects Met when tyrosine phosphorylated. High levels of phospho-Met protein are detected in HCC827 cells where Met is constitutively phosphorylated (Figure 1). These high levels are abolished in HCC827 cells lysed without addition of phosphatase inhibitors* to the lysis buffer. The levels of total Met protein (phospho and nonphospho) as detected by PathScan® Total Met Sandwich ELISA Kit #7242 remain unchanged (Figure 1). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
* Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate and Na3VO4