Description
The PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Kit detects endogenous levels of phospho-S6 ribosomal protein phosphorylated on serines 235/236. As shown in Figure 1, a significant induction of phospho-S6 ribosomal protein (Ser235/236) in PDGF treated NIH/3T3 cells is detected by this kit. However, total S6 ribosomal protein (phospho and non-phospho) remains unchanged. NIH/3T3 or 293 cells treated with 20% FBS after starvation also show similar results (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species