Fig 1: Effect of vicenin 3 and SB203580 on the degradation of ECM in IL-1ß-treated SW1353 human chondrocytes and the p38 MAPK signaling pathway. The protein levels of p-p38 and total p38 were measured by western blot analysis. PGE2 production and protein secretion levels of MMP-1, MMP-3, MMP-13, aggrecan and collagen type II were measured in the culture medium using an ELISA kit. ##P<0.01 and ###P<0.001 compared with control group. *P<0.05, **P<0.01 and ***P<0.001 vs. IL-1ß. ECM, extracellular matrix; IL, interleukin; PGE2, prostaglandin E2; MMP, matrix metalloproteinase.
Fig 2: Effect of vicenin 3 on IL-1ß-induced MMP-1, MMP-3, MMP-13, and ADAMTS-4 and ADAMTS-5 in SW1353 human chondrocytes. (A) The protein secretion levels of MMP-1, MMP-3 and MMP-13 were determined using ELISA. The mRNA expression levels of (B) MMP-1, MMP-3, MMP-13, (C) ADAMTS-4 and (D) ADAMTS-5 were assayed using reverse transcription-quantitative PCR. ###P<0.001 vs. Control group. *P<0.05, **P<0.01 and ***P<0.001 vs. IL-1ß. IL, interleukin; MMP, matrix metalloproteinase; ADAMTS, A disintegrin-like and metalloproteinase with thrombospondin motifs.
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