Fig 1: CSS promoted the expression or secretion of neurotrophic factors, which might facilitate neurogenesis. (A) BDNF and VEGFA expression in hippocampus were detected by Western blotting. The quantitative analysis of BDNF protein (B) and VEGFA protein (C) in hippocampus. (D) BDNF and VEGFA expression were detected in BMVECs by Western blotting. The quantitative analysis of BDNF protein (E) and VEGFA protein (F) in BMVECs. (G and H) The content of BDNF and VEGFA in cell culture supernatant were detected by enzyme-linked immunosorbent assay analysis (n=5). (I) Confocal images to show the distribution and cell number of GLUT1+/BrdU+ cells and NeuN+/BrdU+ in hippocampus. Newborn endothelial cells were labeled by BrdU (red) and GLUT1 (green); newborn neurons were labeled by BrdU (red) and NeuN (blue). White arrows indicate the newborn neurons (n=3). Asterisk represents a statistical difference (*p < 0.05; **p < 0.01).
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