Fig 1: A, A RT-PCR gel electrophoresis image showing the expression of equine POMC (178bp), PC1 (300bp) and PC2 (168bp) in the normal pituitary gland. NC denotes no template control. B, Cycle threshold (Ct) values for the mRNA expression of 18S rRNA and YWHAZ (C) in the pituitary gland of normal (n = 10) and PPID (n = 6) horses. Bars denote mean values (+/- SEM).
Fig 2: POMC (A), PC1 (B), and PC2 (C) mRNA expression in the equine pituitary gland tissue from normal (n = 10) and PPID (n = 6) horses. Bars denote mean values (+/- SEM). *** = p<0.001, ** = p<0.01.
Fig 3: Plasma concentrations of POMC (A), ACTH (B), and a-MSH (C), in normal (n = 6) and PPID (n = 6) horses. Bars denote mean values (+/- SEM). *** = p<0.001.
Fig 4: Partial amino acid sequences for equine POMC (A), PC1 (B), and PC2 (C), compared to predicted Equus caballus and Homo sapiens sequences. Red arrow (A) denotes the cleavage point within POMC for ACTH (the first 13 amino acids of which become a-MSH (red line), and the latter amino acids (18–39) subsequently become CLIP (red arrow with dashed end). Each amino acid is represented by a different color. PC1 and PC2 conserved regions are marked by a red line.
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