Fig 1: Osteogenesis-related differentiation markers of (A) ALP activity (B) BSP, and (C) OC expression of hDPSCs cultured on CA0, CA10, and CA20 scaffolds for different time points. * indicates a significant difference (p < 0.05) from CA0. # indicates a significant difference (p < 0.05) from CA10. Data presented as mean ± SEM; n = 6 for each group.
Fig 2: The relevant indicators of osteogenic and chondrogenic changes observed in WJMSC cultured on LiCS scaffolds over different time points. Osteogenesis in early and late stages markers of (A) ALP expression of WJMSC for 3, 7 and 14 days, and (B) BSP, and (C) OC expression of WJMSC for 7 and 14 days. (D) Alizarin Red S staining of WJMSC to assess calcium mineral deposits after being cultured on scaffolds for 7 and 14 days. (E) Col I concentrations, (F) Col II concentrations, (G) Col II/Col I ratio, and (H) GAG concentrations of Li0, Li5, and Li10 after 3, 7, and 14 days of culture.
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