Fig 1: Schematic illustration of the isolation and purification process of small EVs from murine stool and their characterization. (A) Small EVs (sEV) isolated from the stool of mice from cecum and colon using qEV columns. Analysis of sEV concentration and their size distribution were determined by TRPS and TEM (B,C). Total protein concentration (D) was determined using the Pierce™ 660 nm Protein Assay Kit. Determination of endotoxin amount (E) was performed by Pierce LAL Chromogenic Endotoxin Quantification kit assay, whereas the amount of LTA (F) was measured by Mouse lipoteichoic acids (LTA) ELISA Kit. Protein expression of ß-actin (G) was analyzed by Western blotting. Data are shown as scatter dot plots, mean + SEM (repeated measurements).