Fig 1: AXIN2, TEK knockdown inhibits the proliferation, migration of BRAF ( +) BCPAP cells. A AXIN2, TEK siRNA decreased AXIN2, TEK mRNA expression in BCPAP cells (n = 3). B, C Western blotting analysis of AXIN2, TEK knockdown efficiency in BCPAP cells. D Cell proliferation assay showed that AXIN2, TEK siRNA suppressed the proliferation of BCPAP cells. E After transfection with AXIN2, TEK siRNA or control siRNA, spheroid formation was evaluated at 3 days post-seeding. The spheroid area was measured using ImageJ software, encompassing the entire spheroid to the outside edge of spheroid. Scale bar indicates 1000 µm. F Wound healing assay showed that AXIN2, TEK knockdown resulted in lower migration capacity in BCPAP cells than in negative control cells. Scale bar indicates 1000 µm. (n = 4) Values are means ± SEM *P < 0.05, **P < 0.01, ***P < 0.001
Fig 2: AXIN2, TEK were assayed using an ELISA and immunohistochemistry (Unifocal PTC, n = 42; Multifocal PTC, n = 48). The AXIN2 (A) and TEK (B) values in patients’ serum (median ± SEM). C The receiver operating characteristic (ROC) curve of TEK and AXIN2 in the PTC. D AXIN2 and TEK expression levels were confirmed by immunohistochemistry analysis in tumors (scale bar = 200 µm). Values are means ± SEM ***P < 0.001
Fig 3: Inhibition of miR21, miR34a, miR203, and miR362 in BCPAP cells. A miRNA inhibitor transfection reduced the expression levels of all four miRNAs by more than 40% in BCPAP as analyzed by qPCR. B AXIN2, ADAMTS9 and ADAMTSL2 mRNA expression was up-regulated by miR21 inhibitor (gray), TEK, AXIN2 mRNA expression was up-regulated by miR34a inhibitor (green). And AXIN2, ADAMTS9 and ADAMTSL2 mRNA expression was up-regulated by miR203 inhibitor (orange), AXIN2 mRNA expression was up-regulated by miR362 inhibitor (blue). *P < 0.05, **P < 0.01, ***P < 0.001, compared to the control
Supplier Page from MyBioSource.com for Human Axis Inhibition Protein 2 ELISA Kit