EpiQuik Nuclear Extraction Kit from EpiGentek

Product Specs
ItemEpiQuik Nuclear Extraction Kit
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberOP-0002
Quantity100 Assays
ApplicationsSample Isolation
FormatTube-based
Sample TypeUnisolated Samples
Research AreaChromatin & Transcription, DNA Damage & Repair, DNA Methylation, Gene Expression & Silencing, Phosphorylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

Email: info@epigentek.com
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Company Profile

Website: www.epigentek.com

(1) G6PD Orchestrates Genome-Wide DNA Methylation and Gene Expression in the Vascular WallInternational Journal of Molecular SciencesNovember 24, 2023Christina Signoretti, Sachin A. Guptenitrogen, and nuclear extracts were prepared per the manufacturer’s instructions using a kit (Cat # OP-0002-1) from EpiGentek (Farmingdale, NY, USA). The collected nuclear extracts (20 μg protein) were then used

(2) Host DNA Demethylation Induced by DNMT1 Inhibition Up-Regulates Antiviral OASL Protein during Influenza a Virus InfectionVirusesJuly 28, 2023Zhiyan Zhao, Jing Li, Ye Feng, Xiaoping Kang, Yuchang Li, Yuehong Chen, Wei Li, Wenguang Yang, Lu Zhao, Shenghai Huang, et al.Activity Assay DNMT enzymatic activity was determined using the EpiQuikTM nuclear Extraction kit I (OP-0002, Epigentek, Suffolk County, NY, USA) to extract nuclear extracts from CA07-treated A549 cells. Protein

(3) DNA methylation changes underlie the long-term association between periodontitis and atherosclerotic cardiovascular diseaseFrontiers in Cardiovascular MedicineApril 21, 2023Mohamed Omar, Maria Alexiou, Umar R. Rekhi, Konrad Lehmann, Aneesh Bhardwaj, Cole Delyea, Shokrollah Elahi, Maria FebbraioTET activity assays Nuclear extracts of BMDM were prepared using the EpiQuik Nuclear Extraction Kit (OP-0002-1, Epigentek), following their protocol, generating nuclear and cytoplasmic extracts. The protein concentration

(4) Studies in CRISPR-generated Mediterranean G6PD variant rats reveal G6PD orchestrates genome-wide DNA methylation and gene expression in vascular wallbioRxivMarch 11, 2023Christina Signoretti, Sachin A. Gupteliquid nitrogen, and nuclear extracts were prepared per the manufacturer’s instructions using a kit (OP-0002-1) from EpiGentek (NY, USA). The collected nuclear extracts (20 μg protein) were then used to measure

(5) Therapeutic targeting of histone lysine demethylase KDM4B blocks the growth of castration-resistant prostate cancerBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapieFebruary 1, 2023Duan L, Chen YA, Liang Y, Chen Z, Lu J, Fang Y, Cao J, Lu J, Zhao H, Pong RC, et al.generated using cells treated with B3 or vehicle for 24 h using EpiQuik Nuclear Extraction Kit (Epigentek, OP-0002-1) according to the manufacturer’s instructions and used as the enzyme source to measure demethylase

(6) Dehydroeburicoic Acid, a Dual Inhibitor against Oxidative Stress in Alcoholic Liver DiseasePharmaceuticals (Basel, Switzerland)December 22, 2022Cheng S, Kuang Y, Li G, Wu J, Ko CN, Wang W, Ma DL, Ye M, Leung CH.protocol from the manufacturer (Epigentek, New York, NY, USA, EpiQuik™ Nuclear Extraction Kit I, Catalog # OP-0002), Then, protein expression was analyzed by WB with the indicated antibodies. 4.9. GSK3β Kinase Assay The

(7) Maternal rodent exposure to di‐(2‐ethylhexyl) phthalate decreases muscle mass in the offspring by increasing myostatinJournal of Cachexia, Sarcopenia and MuscleDecember 1, 2022Fengju Li, Ting Luo, Honghui Rong, Lu Lu, Ling Zhang, Chuanfeng Zheng, Dali Yi, Yi Peng, Enyu Lei, Xiaotao Xiong, et al.T9300A, TaKaRa), their nuclear proteins were extracted using the EpiQuik Nuclear Extraction Kit (cat# OP‐0002‐1, Epigentek Farmingdale, NY, USA). The lysates or nuclear proteins (20 μg/lane) were separated by SDS‐PAGE

(8) Cardiac Development in the Presence of Cadmium: An in Vitro Study Using Human Embryonic Stem Cells and Cardiac OrganoidsEnviron Health PerspectNovember 2, 2022Xian Wu, Yichang Chen, Anthony Luz, Guang Hu, Erik J. Tokarextraction buffer (NE2) with DTT and protease inhibitor cocktail using the EpiQuick nuclear extraction kit (OP-0002-1; EPIGENTEK). Total protein content was determined by bicinchoninic acid assay (23225; ThermoFisher Scientific

(9) X-linked myotubular myopathy is associated with epigenetic alterations and is ameliorated by HDAC inhibitionActa NeuropathologicaSeptember 1, 2022Jonathan R. Volpatti et al.mice exposed to PBS or VPA (n = 6/group) were obtained by the EpiQuik™ Nuclear Extraction Kit (cat. OP-0002-1; EpiGentek). Total DNA Methyltransferase (DNMT) activity was measured via calorimetric assay in duplicates

(10) Novel Targeting of DNA Methyltransferase Activity Inhibits Ewing Sarcoma Cell Proliferation and Enhances Tumor Cell Sensitivity to DNA Damaging Drugs by Activating the DNA Damage ResponseFront Endocrinol (Lausanne)May 31, 2022Camilla Cristalli et al.5azadC (3-10 µM) for 48 h, and 5µg of nuclear extracts, isolated with EpiQuick Nuclear Extraction Kit (OP-0002-1, Epigentek Inc), were added to each reaction well, according to the manufacturer’s protocol. Absorbance

(11) Luteolin Causes 5′CpG Demethylation of the Promoters of TSGs and Modulates the Aberrant Histone Modifications, Restoring the Expression of TSGs in Human Cancer CellsInternational Journal of Molecular SciencesApril 6, 2022Sreepoorna Pramodh, Ritu Raina, Arif Hussain, Sali Abubaker Bagabir, Shafiul Haque, Syed Tasleem Raza, Mohammad Rehan Ajmal, Shalini Behl, Deepika BhagavatulaPreparation Nuclear extracts from HeLa cells were prepared using EpiQuikTM nuclear extraction kit (Catalogue number OP-0002; Epigentek, NY, USA) as per the manufacturer’s protocol. Approximately 2 × 106 untreated cells were

(12) Inhibition of Jumonji demethylases reprograms severe dilated cardiomyopathy and prolongs survivalThe Journal of biological chemistryFebruary 1, 2022Tran TA, Zhang QJ, Wang L, Gonzales C, Girard L, May H, Gillette T, Liu ZP, Martinez ED.assays Nuclear extracts were isolated from the left ventricle using EpiQuik Nuclear Extraction Kit (Epigentek; OP-0002-1) according to the manufacturer's instructions. Nuclear extracts were used as the enzyme source to

(13) Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) CellsFrontiers in GeneticsJanuary 12, 2022Ritu Raina, Abdulmajeed G. Almutary, Sali Abubaker Bagabir, Nazia Afroze, Sharmila Fagoonee, Shafiul Haque, Arif Hussainfrom the untreated HeLa cells were prepared using the EpiQuikTM Nuclear Extraction Kit (Catalog No. OP-0002, Epigentek, United States) following the manufacturer’s protocol. The EpiQuik DNMT Activity Assay Kit

(14) METTL3 promotes colorectal carcinoma progression by regulating the m6A-CRB3-Hippo axisJournal of experimental & clinical cancer research : CRJanuary 10, 2022Pan J, Liu F, Xiao X, Xu R, Dai L, Zhu M, Xu H, Xu Y, Zhao A, Zhou W, et al.assay Nuclear extracts of adenoma and CRC tissues were performed using a nuclear extraction kit I (OP-0002, Epigentek, USA). Briefly, tissues were cut into small pieces, and homogenized. Then they were incubated

(15) Toll-like receptor 4 mutation protects the kidney from Ang-II-induced hypertensive injuryPharmacological researchJanuary 1, 2022Majumder S, Pushpakumar S, Juin SK, Jala VR, Sen U.guidelines. Nuclear fraction isolation was performed by Epiquik nuclear extraction kit (Epigentek, OP-0002-1). 2.6. Immunoblot analysis Kidney homogenates were separated by SDS-PAGE and transferred to Polyvinylidene

(16) Adipocyte-mediated epigenomic instability in human T-ALL cells is cytotoxic and phenocopied by epigenetic-modifying drugsFrontiers in cell and developmental biologyJanuary 1, 2022Lee M, Geitgey DK, Hamilton JAG, Boss JM, Scharer CD, Spangle JM, Haynes KA, Henry CJ.harvested and washed in 1X PBS. Nuclear extracts were prepared, following the manufacturer’s protocol (Cat#OP-0002-1, Epigentek, Farmingdale, NY, United States), and 5 μg was used to measure either HAT (Cat#P-4003,

(17) Lung Allograft Epithelium DNA Methylation Age Is Associated With Graft Chronologic Age and Primary Graft DysfunctionFrontiers in ImmunologyOctober 7, 2021Daniel T. Dugger et al.trypsin neutralization with FBS, centrifugation, and enumeration. The Epigentek Nuclear Extraction Kit (OP-0002-1) was used to isolate nuclear proteins. Protein concentration was determined by bicinchoninic acid

(18) The Small Molecule BC-2059 Inhibits Wingless/Integrated (Wnt)-Dependent Gene Transcription in Cancer through Disruption of the Transducin β-Like 1-β-Catenin Protein ComplexJournal of Pharmacology and Experimental TherapeuticsAugust 1, 2021Raffaella Soldi et al.analysis Nuclear extracts were prepared from HCT15 cells using the Epigentek nuclear extraction kit (catalog no. OP-0002-1) according to the manufacturer's protocol. The total protein 10 Downloaded from jpet.aspetjournals.org

(19) Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal PathwayACS omegaJanuary 12, 2021Dong Q, Li Y, Chen J, Wang N.Scientific, Waltham). The nuclear protein was isolated using an EpiQuik Nuclear Extraction Kit (Cat#OP-0002, EpiGentek). The expression of NF-κB p65 was measured using nuclear fragmentations. The expressions

(20) Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densitiesPLOS ONEJanuary 7, 2021Daniel F. Clark, Rachael Schmelz, Nicole Rogers, Nuri E. Smith, Kimberly R. Shorteractivity assays Nuclear extracts were isolated using the Nuclear Extraction Kit (Epigentek; Cat. No. OP-0002-1). Three replicates were obtained per treatment group; each replicate contained the contents of twelve

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