EpiQuik Total Histone Extraction Kit from EpiGentek

Product Specs
ItemEpiQuik Total Histone Extraction Kit
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberOP-0006
Quantity100 Extractions
ApplicationsSample Isolation
FormatMicroplate-based
Sample TypeUnisolated Samples
Research AreaHistone Acetylation, Histone Methylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

Email: info@epigentek.com
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Company Profile

Website: www.epigentek.com

(1) Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cellsiScienceJanuary 19, 2024Zhe Cheng, Huichao Huang, Maoyu Li, Yongheng Chenusing the EpiQuik Total Histone Extraction Kit according to the manufacturer’s instructions (Epigentek, OP-0006-100). Briefly, cells were collected and resuspended in pre-lysis buffer containing protease inhibitor

(2) Asymmetric and symmetric protein arginine methylation in methionine-addicted human cancer cellsPLoS OneDecember 22, 2023Ashley G. Holtz, Troy L. Lowe, Yusuke Aoki, Yutaro Kubota, Robert M. Hoffman, Steven G. Clarke4°C for 5 min. Histone extraction The EpiQuick Total Histone Extraction Kit (EpiGentek, catalog #OP-0006) was used to extract the histones from each cell line. One aliquot (250,000 cells for H460 and HCT116,

(3) The SMYD3-dependent H3K4me3 status of IGF2 intensifies local Th2 differentiation in CRSwNP via positive feedbackCell Communication and SignalingNovember 30, 2023Lei Yu, Yi Wei, Tong Lu, Zhengqi Li, Shimin Lai, Yan Yan, Changhui Chen, Weiping Wenthe EpiQuik™ Total Histone Extraction Kit was used according to the manufacturer's protocol (Cat # OP-0006, EpiGentek). Th2 and non-Th2 CRSwNP diagnoses were made based on the levels of Th cytokines, including

(4) Reducing branched-chain amino acids improves cardiac stress response in mice by decreasing histone H3K23 propionylationJournal of Clinical InvestigationNovember 15, 2023Zhi Yang, Minzhen He, Julianne Austin, Danish Sayed, Maha Abdellatifprotocols. Histones were extracted using EpiGentek’s EpiQuik Total Histone Extraction Kit (catalog OP-0006-100). The cellular fractions were separated on a 4%–12% gradient SDS-PAGE (Criterion gels, Bio-Rad)

(5) A DNA adenine demethylase impairs PRC2-mediated repression of genes marked by a specific chromatin signatureGenome BiologyAugust 30, 2023Qingxiao Jia, Xinran Zhang, Qian Liu, Junjie Li, Wentao Wang, Xuan Ma, Bo Zhu, Sheng Li, Shicheng Gong, Jingjing Tian, et al.using The EpiQuik™ Total Histone Extraction Kit by following the manufacturer’s instruction (Epigentek; OP-0006-100) and then detected by western blot with anti-H3 (Abcam; ab1791), anti-H3K27me3 (Millipore; 07-449),

(6) In Vivo Identification of H3K9me2/H3K79me3 as an Epigenetic Barrier to CarcinogenesisInternational Journal of Molecular SciencesJuly 29, 2023Maria Cristina Piro, Valeria Gasperi, Alessandro De Stefano, Lucia Anemona, Claudio Raffaele Cenciarelli, Manuela Montanaro, Alessandro Mauriello, Maria Valeria Catani, Alessandro Terrinoni, Alessandra Gambacurtawere isolated by using EpiQuik Total Histone Extraction Kit (Epigentek, Farmingdale, NY, USA, cod. OP-0006), according to manufacturer instructions. Briefly, cell pellets were resuspended in 1 mL of Diluted

(7) An enhanced network of energy metabolism, lysine acetylation, and growth-promoting protein accumulation is associated with heterosis in elite hybrid ricePlant CommunicationsJuly 10, 2023Xuan Ma, Qingxiao Jia, Sheng Li, Zhengting Chen, Xin Ming, Yu Zhao, Dao-Xiu Zhouextracted from the frozen panicle samples using the EpiQuik Total Histone Extraction kit (Epigentek, Cat.# OP-0006) following the protocol. Concentration of the eluted protein was measured by the Bradford method according

(8) ACL and HAT1 form a nuclear module to acetylate histone H4K5 and promote cell proliferationNature CommunicationsJune 5, 2023Qiutao Xu, Yaping Yue, Biao Liu, Zhengting Chen, Xuan Ma, Jing Wang, Yu Zhao, Dao-Xiu Zhoufile. Histone extraction and western blot The EpiQuik Total Histone Extraction Kit (Epigentek USA, OP-0006-100) was used to extract total histone-enriched fractions from 6 DAF rice seeds or seven-day-old rice

(9) Maternal immune activation alters placental histone-3 lysine-9 tri-methylation, offspring sensorimotor processing, and hypothalamic transposable element expression in a sex-specific mannerNeurobiology of StressMay 1, 2023Holly DeRosa, Arianna Smith, Laurel Geist, Ada Cheng, Richard G. Hunter, Amanda C. Kentner24 h after maternal immune activation according to the manufacturer's instructions (Epigentek, Cat. #OP-0006-100). Protein concentrations of each extract was determined using the BCA assay (Pierce™, Cat. #23227).

(10) CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growthCell reportsApril 25, 2023Yin S, Liu L, Ball LE, Wang Y, Bedford MT, Duncan SA, Wang H, Gan W.NE-PER™ Nuclear and Cytoplasmic Extraction Reagents,Thermo Fisher,Cat# 78833 EpiQuik Total Histone Extraction Kit,Epigentek,Cat# OP-0006–100 Experimental models: Cell lines,, Human: HEK293,ATCC,Cat# CRL-1573 Human: HEK293T,ATCC,Cat# CRL-3216 Human:

(11) Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changesWellcome Open ResearchMarch 30, 2023Gilda Padalino, Cassandra A. Celatka, Hugh Y. Rienhoff Jr., Jay H. Kalin, Philip A. Cole, Damien Lassalle, Josephine Forde-Thomas, Iain W. Chalmers, Andrea Brancale, Christoph Grunau, et al.TissueLyser (Qiagen) and total histones were extracted using the EpiQuikTM Total Histone Extraction kit (OP-0006, Epigentek) following the manufacturer’s instructions. A total of 2.5–10 µg of each sample was separated

(12) Putative SET-domain methyltransferases in Cryptosporidium parvum and histone methylation during infectionVirulenceDecember 31, 2022Manasi Sawant et al.were subjected to histone purification using the EpiQuikTM Total Histones Extraction Kit (Epigentek, OP-0006-100, USA). The purified histone concentration was determined using micro BCA protein assay kit (Pierce,

(13) H3K18 lactylation marks tissue-specific active enhancersGenome BiolOctober 3, 2022Eva Galle et al.types. Western blot Histone extracts were prepared with the EpiQuik Total Histone Extraction kit (Epigentek, OP-0006-100-EP; for MB, MT, and GAS) or the acid histone extraction protocol published by Abcam (mESC, ADIPO,

(14) Single-cell sequencing reveals activation of core transcription factors in PRC2-deficient malignant peripheral nerve sheath tumorCell ReportsSeptember 20, 2022Xiyuan Zhang, Hannah E. Lou, Vishaka Gopalan, Zhihui Liu, Hilda M. Jafarah, Haiyan Lei, Paige Jones, Carly M. Sayers, Marielle E. Yohe, Prashant Chittiboina, et al.BioScience,Cat# 4515 Bradford Assay Dye,Bio-Rad,Cat# 5000006 Total Histone Extraction Kit,Epigentek,OP-0006-100 Dynabeads M-280 Sheep anti-Rabbit IgG Magnetic Beads,Invitrogen,Cat# 11203D RNeasy Mini Kit,Qiagen,Cat#

(15) Trained Immunity Enhances Human Monocyte Function in Aging and SepsisFrontiers in ImmunologyMay 25, 2022P. Spencer Gill, Tammy R. Ozment, Nicole H. Lewis, Edward R. Sherwood, David L. Williamsat day 3. On day 7, histones were isolated using the EpiQuik Total Histone Extraction Kit (EpiGentek OP–0006–100) and frozen at –80°C. H3K27 acetylation was assayed using the EpiQuik Global Acetyl Histone H3K27

(16) Linkage of methionine addiction, histone lysine hypermethylation, and malignancyiScienceApril 15, 2022Yamamoto J, Inubushi S, Han Q, Tashiro Y, Sugisawa N, Hamada K, Aoki Y, Miyake K, Matsuyama R, Bouvet M, et al.assays,Critical commercial assays,Critical commercial assays ,, EpiQuik Total Histone Extraction Kit,Epigentek,#OP-0006 Cell Counting Kit-8,Dojindo,CK04 Histofine SAB-PO® kit,Nichirei,03AM0770 Clarity Western ECL Substrate,Bio-Rad,#1705060 ,, Experimental models

(17) Reversion of methionine addiction of osteosarcoma cells to methionine independence results in loss of malignancy, modulation of the epithelial-mesenchymal phenotype and alteration of histone-H3 lysine-methylationFrontiers in oncologyJanuary 1, 2022Aoki Y, Han Q, Tome Y, Yamamoto J, Kubota Y, Masaki N, Obara K, Hamada K, Wang JD, Inubushi S, et al.Thermo Fisher Scientific). Extraction of total histones used an Epiquik Total Histone Extraction Kit (#OP-0006-100, Epigentek, Farmingdale, NY, USA). Western immunoblotting for total proteins and histones was performed

(18) The long noncoding RNA H19 regulates tumor plasticity in neuroendocrine prostate cancerNature communicationsDecember 21, 2021Singh N, Ramnarine VR, Song JH, Pandey R, Padi SKR, Nouri M, Olive V, Kobelev M, Okumura K, McCarthy D, et al.cultures with post-translational modifications intact, EpiQuik Total Histone Extraction Kit (Epigentek, OP-0006-100) was used according to the manufacturer’s protocol. Antibodies used: SOX2 (sc-365823, Santa Cruz Biotechnology

(19) Molecular profiling of epigenetic landscape of cancer cells during extracellular matrix detachmentScientific ReportsDecember 1, 2021Mohammad Imran Khan, Mazin A. Zamzami, Aftab Ahmad, Hani Choudhrywith the use of a commercial kit Epigentek Inc., U.S.A. (EpiQuik™ Total Histone Extraction Kit cat no: OP-0006-100). Briefly, 2–3 × 107 million cells were utilized for histone isolation, and their protein contents

(20) VLX600 Disrupts Homologous Recombination and Synergizes with PARP Inhibitors and Cisplatin by Inhibiting Histone Lysine DemethylasesMolecular Cancer TherapeuticsSeptember 1, 2021Thomas L. Ekstrom, Nicholas M. Pathoulas, Amelia M. Huehls, Arun Kanakkanthara, Larry M. Karnitzto clean microcentrifuge tubes. Histone extracts were prepared via supplier's protocol (Catalog No. OP-0006, EpiGentek). Protein concentrations were determined using the Bio-Rad Bradford Assay (Catalog No. 5000006).

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