ERK1/2 (pT202/Y204 + Total) ELISA Kit ab176660 from Abcam

Product Specs
ItemERK1/2 (pT202/Y204 + Total) ELISA Kit
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab176660
Quantity1 96TESTS
ReactivityHuman, Mouse
ApplicationsELISA
Gene NameMAPK1
TypeSemi-quantitative
NCBI Gene AliasesERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Detection TargetERK1+ERK2

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(1) AXL receptor tyrosine kinase modulates gonadotropin-releasing hormone receptor signalingCell Communication and SignalingOctober 12, 2023Pardis Mohammadzadeh, Mina Roueinfar, Gregory C. Ambergplotted as histograms. ERK phosphorylation measurements Semiquantitative sandwich ELISA kit (Abcam, ab176660) was used to measure ERK phosphorylation (pERK) and total ERK (pERK/ERK) in LβT2 and αT3-1 cells in

(2) Regulation of Soluble E-Cadherin Signaling in Non-Small-Cell Lung Cancer Cells by Nicotine, BDNF, and β-Adrenergic Receptor LigandsBiomedicinesSeptember 18, 2023Ravel Ray, Stuti Goel, Hind Al Khashali, Ban Darweesh, Ben Haddad, Caroline Wozniak, Robert Ranzenberger, Jeneen Khalil, Jeffrey Guthrie, Deborah Heyl, et al.Signal-Regulated Kinase (ERK) Assay The ERK1/2 (pT202/Y204 + Total) ELISA kit (Abcam, Waltham, MA, USA, ab176660) was used to quantitate ERK according to the instructions provided by the manufacturer and our previously

(3) Erk1/2-Dependent HNSCC Cell Susceptibility to Erastin-Induced FerroptosisCellsJanuary 16, 2023Dragana Savic, Teresa Bernadette Steinbichler, Julia Ingruber, Giulia Negro, Bertram Aschenbrenner, Herbert Riechelmann, Ute Ganswindt, Sergej Skvortsov, József Dudás, Ira-Ida Skvortsovatotal ERK1/2 proteins, the Erk1 (phospho T202 + Y204) + Erk2 (phospho T185 + Y187) + Total ELISA Kit (ab176660; Abcam, UK) was used. Cells were treated with ravoxertinib, erastin, or their combination as described

(4) Regulation of the Soluble Amyloid Precursor Protein α (sAPPα) Levels by Acetylcholinesterase and Brain-Derived Neurotrophic Factor in Lung Cancer Cell MediaInternational Journal of Molecular SciencesSeptember 15, 2022Hind Al Khashali, Ravel Ray, Kai-Ling Coleman, Sarah Atali, Ben Haddad, Jadziah Wareham, Jeffrey Guthrie, Deborah Heyl, Hedeel Guy EvansATP. 4.9. ERK Assay ERK quantitation was measured using the ERK1/2 (pT202/Y204 + Total) ELISA kit (Abcam, ab176660) according to the instructions provided by the manufacturer. In brief, cell lysate samples were added

(5) BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguardingProtein & cellAugust 1, 2022Wang M, Zhao K, Liu M, Wang M, Qiao Z, Yi S, Jiang Y, Kou X, Zhao Y, Yin J, et al.(ELISA) We performed ELISA following the manufacturer’s instructions (ERK1/2 (pT202/Y204 + Total) ELISA Kit, ab176660, Abcam). Each group has three experiment replicates, and each replicate was performed using 25,000 cells.

(6) FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 PathwayBalkan Medical JournalJuly 25, 2022Yang Li, Shulan DouAbcam), p-MEK (1 µg/ml, ab278716, Abcam), ERK1/2 (1/10000, ab184699 Abcam), and p-ERK1/2 (0.2 ng/ml, ab176660, Abcam). Transwell Assay The migration of CC cells was examined in a 24-well Transwell plate (Costar).

(7) The SARS-CoV-2 Spike Protein Disrupts the Cooperative Function of Human Cardiac Pericytes - Endothelial Cells Through CD147 Receptor-Mediated Signalling: A Potential Non-Infective Mechanism of COVID-19 Microvascular DiseaseSSRN Electronic JournalMarch 29, 2022Elisa Avolio, Monica Gamez, Kapil Gupta, Rebecca Foster, Imre Berger, Massimo Caputo, Andrew Davidson, Darryl Hill, Paolo Madedducardiac PCs, the ERK1/2 ELISA® Kit was employed, according to the manufacturer’s instructions (Abcam ab176660). PCs were pre-incubated with antiACE2 (20 μg/mL, as described before5) or anti CD147 (20 μg/mL) antibodies

(8) Vincamine Modulates the Effect of Pantoprazole in Renal Ischemia/Reperfusion Injury by Attenuating MAPK and Apoptosis Signaling PathwaysMoleculesFebruary 18, 2022Michael A. Fawzy, Sherif A. Maher, Mahmoud A. El-Rehany, Nermeen N. Welson, Nisreen K. A. Albezrah, Gaber El-Saber Batiha, Moustafa Fathyab207483), phosphor-ERK (1:1000, Abcam, Cambridge, UK, #Ab207470), ERK (1:1000, Abcam, Cambridge, UK, #ab176660), NF-kB p65 (1:5000, Abcam, Cambridge, UK, #Ab32536), JNK (1:1000, Abcam, Cambridge, UK, #Ab179461),

(9) The SARS-CoV-2 Spike protein disrupts human cardiac pericytes function through CD147 receptor-mediated signalling: a potential non-infective mechanism of COVID-19 microvascular diseaseClinical science (London, England : 1979)December 22, 2021Avolio E, Carrabba M, Milligan R, Kavanagh Williamson M, Beltrami AP, Gupta K, Elvers KT, Gamez M, Foster RR, Gillespie K, et al.detection of ERK1/2 phosphorylation in cardiac PCs, either Western blotting or the ERK1/2 ELISA® Kit (Abcam ab176660) were employed. PCs were cultured with EBM2 medium under FBS and growth factors deprivation for 24 h.

(10) Cervical carcinoma high-expressed long non-coding RNA 1 promotes papillary thyroid carcinoma cell proliferation and invasionTranslational Cancer ResearchSeptember 1, 2021Ping Shi, Yan Liu, Mingzeng Zhang, Jianwang Yang, Shanghua Jing, Dongqiang Yang, Fei Liu, Yanzhao Wu, Huijing Shi, Cuizhi Gengwere incubated 1:1,000 dilutions of the following primary antibodies: anti-ERK (ab32537), anti-p-ERK (ab176660), anti-p38 MAPK (ab197348), anti-p-p38 MAPK (ab176664), and anti-microtubule protein (ab11304) (Abcam,

(11) Combinatorial allosteric modulation of agonist response in a self-interacting G-protein coupled receptorCommunications BiologyDecember 1, 2020Marco Patrone, Eugenia Cammarota, Valeria Berno, Paola Tornaghi, Davide Mazza, Massimo Deganodescribed in the text and then processed using the ERK1/2 (pT202/Y204 + Total) ELISA Kit from Abcam (cat n. ab176660), following the manufacturer guidelines and adding the HALT™ Proteases & Phosphatases inhibitor cocktail

(12) Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK AxisMedical science monitor : international medical journal of experimental and clinical researchAugust 30, 2020Guo T, Liu Y, Ren X, Wang W, Liu H.tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b) were detected using ELISA kits (ERK/MEK, ab176660, Abcam; TNF-a, KE10002, Proteintech Group, Inc., Wuhan, Hubei, China; IL-1b, KE10003, Proteintech) in

(13) NEAT1 Negatively Regulates Cell Proliferation and Migration of Neuroblastoma Cells by miR-183-5p/FOXP1 Via the ERK/AKT PathwayCell TransplantationJuly 23, 2020Weikang Pan, Ali Wu, Hui Yu, Qiang Yu, Baijun Zheng, Weili Yang, Donghao Tian, Ya Gao, Peng Liwere, respectively, used to incubate with the membrane at 4°C overnight: ERK antibody (1:500 dilution, ab176660, Abcam, Cambridge, UK), AKT antibody (1:400 dilution, ab142088, Abcam), S6 antibody (1: 300 dilution,

(14) Sustained release and protein stabilization reduce the growth factor dosage required for human pluripotent stem cell expansionBiomaterialsJuly 1, 2020Andrew S. Khalil, Angela W. Xie, Hunter J. Johnson, William L. Murphyfor measuring bFGF (Quantikine basic FGF ELISA, R&D Systems, DFB50), phosphoERK and total ERK (Abcam, ab176660) were performed following the manufacturer’s recommended protocol. MCMs were confirmed not to interfere

(15) Factor XII blockade inhibits aortic dilatation in angiotensin II-infused apolipoprotein E-deficient miceClinical ScienceMay 15, 2020Corey S. Moran, Sai-Wang Seto, Erik Biros, Smriti M. Krishna, Susan K. Morton, Christoph Kleinschnitz, Con Panousis, Jonathan GolledgeSimpleStep ELISA™, Abcam), and total and phosphorylated extracellular signal-regulated kinase (Erk1/2, ab176660, SimpleStep ELISA™, Abcam). Tumor necrosis factor-α-converting enzyme (TACE/a disintegrin and metalloproteinase-17

(16) Therapeutic contribution of melatonin to the treatment of septic cardiomyopathy: A novel mechanism linking Ripk3-modified mitochondrial performance and endoplasmic reticulum functionRedox BiologySeptember 1, 2019Jiankai Zhong et al.Cell Signaling Technology, #3682), t-Akt (1:1000, Abcam, #ab8805), p-Akt (1:1000, Abcam, #ab81283), p-ERK (1:1000, Abcam, #ab176660), t-ERK (1:1000, Abcam #ab54230), PERK (1:1000, Abcam, #ab79483), CHOP (1:1000, Abcam, #ab179823), GRP78

(17) Liraglutide protects renal mesangial cells against hyperglycemia‑mediated mitochondrial apoptosis by activating the ERK‑Yap signaling pathway and upregulating Sirt3 expressionMolecular medicine reportsApril 1, 2019Li J, Li N, Yan S, Lu Y, Miao X, Gu Z, Shao Y.antibodies used in the present study were as follows: Phosphor ylated (p)‑ERK (1:1,000; Abcam; cat. no., ab176660), Sirt3 (1:1,000; Abcam; cat. no., ab86671) and cytochrome‑c (Cyt‑c; 1:1,000; Abcam; cat. no., ab90529).

(18) Human TNF-Luc reporter mouse: A new model to quantify inflammatory responsesScientific reportsJanuary 17, 2019Minshawi F, White MRH, Muller W, Humphreys N, Jackson D, Campbell BJ, Adamson A, Papoutsopoulou S.detection of ERK phosphorylation and total ERK protein, the ERK1/2 (pT202/Y204 + Total) ELISA Kit (ab176660; AbCam, UK) was used. For detection of p65 DNA-binding activity, the TransAm Flexi NFκB p50/NFκB p65

(19) TNFα promotes glioblastoma A172 cell mitochondrial apoptosis via augmenting mitochondrial fission and repression of MAPK–ERK–YAP signaling pathwaysOncoTargets and TherapyNovember 15, 2018Changyu Lu, Xiaolei Chen, Qun Wang, Xinghua Xu, Bainan XuHealthcare, Buckinghamshire, UK). The primary antibodies used in the present study are described as follows: p-ERK (1:1,000, #ab176660; Abcam, Cambridge, MA, USA), t-ERK (1:1,000, #ab54230; Abcam), Yap (1:1,000; #14074; Cell Signaling Technology

(20) Therapeutic effect of Sirtuin 3 on ameliorating nonalcoholic fatty liver disease: The role of the ERK-CREB pathway and Bnip3-mediated mitophagyRedox biologySeptember 1, 2018Li R, Xin T, Li D, Wang C, Zhu H, Zhou H.Cell Signalling Technology, #4599), Atg5 (1:1000, Cell Signalling Technology, #12994), p-ERK (1:1000, Abcam, #ab176660), t-ERK (1:1000, Abcam #ab54230), cyt-c (1:1000; Abcam; #ab90529), c-IAP (1:1000, Cell Signalling Technology,

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ERK1/2 (pT202/Y204 + Total) ELISA Kit from Abcam

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ERK1/2 (pT202/Y204 + Total) ELISA Kit from Abcam

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