Fig 1: Gene expression analysis is relative to GAPDH of AFP, ALB, KRT19, and MKI67 at various time points. All experiments were conducted in triplicate. Error bars show the standard error of the mean (* p < 0.05 and **** p < 0.0001).
Fig 2: Expression of liver-specific functions in (A) co-culture of HepG2/NIH 3T3 cells compared to only HepG2 cells with Albumin secretion in µg/mL/105 cells (n = 3, * p < 0.05 and ** p < 0.01) and gene expression analysis of (B) HepG2/NIH 3T3 cells (n = 3, * p < 0.05 and ** p < 0.01) compared to (C) HepG2 cells (n = 3, ** p < 0.01, *** p < 0.001 and **** p < 0.0001) at day 1, 3, and 7 for liver-specific genes AFP, ALB, KRT19, and MKI67. Error bars represent the standard error of the mean.
Fig 3: Hepatic function evaluations of 2D and 3D models. (A) Immunostaining of ALB (green) and nuclei (bule, DAPI) of cells on Day 7 during culture period. Determination of the total (B) ALB and (C) urea secretions in the supernatant at days 1, 4 and 7 during culture. The asterisks in figure indicate the level of significant difference (*p < 0.05, **p < 0.01).
Supplier Page from Abcam for Human Albumin ELISA Kit