Fig 1: Expression of NAFLD phenotype in 2-chamber systems. Oil Red O staining showing lipid accumulation in hepatocytes after 14 days in recirculating 2-chamber systems containing (a) Healthy BMM (b) Diabetic BMM (c) Diabetic + obese BMM (d) Proinflammatory BMM (e) Diabetic + proinflammatory BMM (f) Diabetic, obese + proinflammatory BMM). (g) Quantification of hepatocyte lipid accumulation. ICC quantification of insulin resistance biomarkers after 14d in recirculating (h) Healthy BMM (i) Diabetic BMM (j) Diabetic + Obese BMM (k) Diabetic, obese, and proinflammatory BMM. Statistical analysis performed as multi-way ANOVA followed by Dunnett’s test (a = 0.05) using the coverslip-wide average of two independent coverslips per condition.
Fig 2: Characterization of adipocytes in monoculture. (a) Cytometer live/dead, FABP4, and CD200 staining of adipocytes after 14d differentiation. Oil Red O staining showing lipid storage in adipocytes after differentiation and 14-day treatment in (b) Healthy BMM (c) Diabetic BMM (d) Obese BMM (e) Proinflammatory BMM (f) Diabetic + proinflammatory BMM (g) Obese + proinflammatory BMM). (h) Quantification of lipid droplet size using ImageJ particle analysis. ICC quantification of insulin resistance biomarkers after 14d in (i) Healthy BMM (j) Diabetic BMM (k) Diabetic + Obese BMM (l) Diabetic, obese, and proinflammatory BMM. Statistical analysis performed as multi-way ANOVA followed by Dunnett’s test (a = 0.05) using the coverslip-wide average of two independent coverslips per condition.
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