Description
LabAssay™ Creatinine is a kit used for the determination of creatinine in blood (serum, plasma) and urine using the Jaffé reaction. With the use of a microplate, this kit provides a convenient method for measuring creatinine in samples.The Jaffé reaction is widely used for creatinine measurement because of its simple procedures and a high degree of coloration.Creatinine is a metabolic product formed by the dehydration and cyclization of creatine and is a type of non-protein nitrogen. Phosphorylated creatine (creatine phosphate) is an important energy source for muscle contraction, and creatinine, a metabolic product of creatine, is produced mainly in the muscles. In humans, the daily conversion rate from creatine to creatinine is almost constant. Unlike the biochemically similar test item, blood urea nitrogen (BUN), the amount of creatinine in the serum is not affected by protein intake. On the other hand, muscle mass and the level of exercise influence creatinine production.The produced creatinine is excreted in the urine via the kidneys. The serum creatinine level rises when kidney function is compromised. The creatinine level increases significantly when about 70% of the nephrons in both kidneys become dysfunctional. Conversely, the serum creatinine level often remains within the normal range in cases of mild to moderate kidney dysfunction, making it an inappropriate indicator for early kidney dysfunction