HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a systemiScienceDecember 15, 2023Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung YuDNA Assembly Cloning Kit,New England Biolabs,Cat# E5520S T7 RNA Polymerase,New England Biolabs,Cat# E2040S SP6 RNA Polymerase,New England Biolabs,Cat# E2070S Anti-Reverse Cap Analog (ARCA),New England Biolabs,Cat#

(2) Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryosMolecular Therapy - Nucleic AcidsDecember 12, 2023Weikai Ge, Xiaozhu Zhao, Shixue Gou, Qin Jin, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, et al.using the HiScribe T7 ARCA mRNA Kit (NEB, E2060S) and HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) and harvested RNA using the RNeasy MinElute Cleanup Kit (Qiagen, 12243). The final concentrations of

(3) The ribosome‐associated chaperone Zuo1 controls translation upon TORC1 inhibitionThe EMBO JournalDecember 11, 2023Ailsa Black, Thomas D Williams, Flavie Soubigou, Ifeoluwapo M Joshua, Houjiang Zhou, Frederic Lamoliatte, Adrien RousseauCoprecipitant,Thermo Fisher,Cat #AM9515 HiScribe T7 High Yield RNA synthesis Kit,New England Biolabs,Cat#E2040S Critical commercial assays,Critical commercial assays,Critical commercial assays RNeasy Mini Kit,Qiagen,Cat

(4) Purification and functional validation of LtCas12a proteinSTAR ProtocolsDecember 1, 2023Bo Zhou, Ye Chen, Lifang Li, Jiashuo Liu, Yuyan Wang, Zheying Huang, Zheng Hu, Rui Tian, Zhen Liassays ,, SDS-PAGE Gel Preparation Kit,Yeasen,Cat#20328ES50 HiScribe T7 High Yield RNA Synthesis Kit,NEB,Cat#E2040S Monarch RNA Cleanup Kit,NEB,Cat#T2040L EasyPure® Quick Gel Extraction Kit,Trans,Cat#EG101-01 Lateral

(5) Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probesNature CommunicationsNovember 14, 2023Ren Ren, Shenglin Cai, Xiaona Fang, Xiaoyi Wang, Zheng Zhang, Micol Damiani, Charlotte Hudlerova, Annachiara Rosa, Joshua Hope, Nicola J. Cook, et al.amplicons were transcribed to single-stranded RNA using HiScribeTM T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs). 5 µl of DNA amplicon products were mixed with 1.5 µl of T7 reaction buffer (10×),

(6) Proenkephalin-A secreted by renal proximal tubules functions as a brake in kidney regenerationNature CommunicationsNovember 7, 2023Chi Liu, Xiaoliang Liu, Zhongwei He, Jiangping Zhang, Xiaoqin Tan, Wenmin Yang, Yunfeng Zhang, Ting Yu, Shuyi Liao, Lu Dai, et al.PCR amplification. Subsequently, the gRNA was synthesized using a Hiscribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). For CRISPR/Cas9 mutagenesis, a mixed solution containing 1 nL of Cas9 protein (0.7 μM, NEB, M0646T)

(7) Genome-wide analysis of the interplay between chromatin-associated RNA and 3D genome organization in human cellsNature CommunicationsOctober 16, 2023Riccardo Calandrelli, Xingzhao Wen, John Lalith Charles Richard, Zhifei Luo, Tri C. Nguyen, Chien-Ju Chen, Zhijie Qi, Shuanghong Xue, Weizhong Chen, Zhangming Yan, et al.in vitro transcription by T7 RNA polymerase, using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs-E2040S) following the manufacturer’s instructions. Resulting transcription reactions were treated with DNAse

(8) Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality controlbioRxivOctober 4, 2023Katharine Y. Chen, Heungwon Park, Arvind Rasi Subramaniamcassette, similar to previous approaches27,86, using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S). Transcribed gRNA was cleaned using the RNA Clean and Concentrator kit (Zymo R1013). Library mRNA

(9) Click display: a rapid and efficient in vitro protein display method for directed evolutionNucleic Acids ResearchSeptember 8, 2023Yu Zeng, Michael Woolley, Karuppiah Chockalingam, Benjamin Thomas, Srishtee Arora, Magnus Hook, Zhilei Chensynthesis (1 μg per 20 μl reaction, HiScribe™ T7 High-Yield RNA Synthesis Kit (New England Biolabs, E2040S)). At the end of the reaction, 70 μl nuclease-free water, 10 μl 10× DNase I Buffer, and 2 μl DNase I

(10) Programmable RNA detection with CRISPR-Cas12aNature CommunicationsSeptember 5, 2023Santosh R. Rananaware, Emma K. Vesco, Grace M. Shoemaker, Swapnil S. Anekar, Luke Samuel W. Sandoval, Katelyn S. Meister, Nicolas C. Macaluso, Long T. Nguyen, Piyush K. Jainamplified product was in-vitro transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) following the manufacturer’s protocol. The transcribed product was treated with DNase I for 30 min

(11) An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragmentsSTAR ProtocolsSeptember 1, 2023Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, et al.generate crRNA pairs (Figure 2). NEB HiScribeTM T7 Quick High Yield RNA Synthesis Kit (NEB, Catalog# E2040S) is used in this step.a.Prepare 100 μL in vitro transcription master mix (see materials and equipment)

(12) Single-cell massively-parallel multiplexed microbial sequencing (M3-seq) identifies rare bacterial populations and profiles phage infectionNature MicrobiologyAugust 31, 2023Bruce Wang, Aaron E. Lin, Jiayi Yuan, Katherine E. Novak, Matthias D. Koch, Ned S. Wingreen, Britt Adamson, Zemer GitaiSPRI and used for in vitro transcription. Guide RNAs were transcribed using the NEB HiScribe kit (NEB E2040S) by mixing 100 ng of DNA template, 2 μl of 10× reaction buffer, 2 μl 100 mM ATP, 2 μl 100 mM GTP, 2

(13) The non-redundant functions of PIWI family proteins in gametogenesis in golden hamstersNature CommunicationsAugust 29, 2023Xiaolong Lv, Wen Xiao, Yana Lai, Zhaozhen Zhang, Hongdao Zhang, Chen Qiu, Li Hou, Qin Chen, Duanduan Wang, Yun Gao, et al.and amplify sgRNAs in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). The complementary target oligonucleotides were cloned into the pUC57-T7-sgRNA-trcRNA vector through

(14) In vitro Analysis of Stalled Ribosomes Using Puromycin IncorporationBio ProtocAugust 20, 2023MaKenzie R. Scarpitti, Michael G. Kearsecatalog number: 4401457)HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, catalog number: E2040S)iScript Reverse Transcription Supermix (Bio-Rad, catalog number: 1708841)iTaq Universal SYBR Green Supermix,

(15) Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV‐1 pathogenesisThe EMBO JournalAugust 15, 2023Yetao Wang, Lawrence Lifshitz, Noah J Silverstein, Esther Mintzer, Kevin Luk, Pamela StLouis, Michael A Brehm, Scot A Wolfe, Steven G Deeks, Jeremy Lubanand subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), then treated with ExoSAP‐IT (Affymetrix, 78200). IVT RNA was fragmented using RNA fragmentation reagents

(16) Multiple TMA-aided CRISPR/Cas13a platform for highly sensitive detection of IL-15 to predict immunotherapeutic response in nasopharyngeal carcinomaJ Immunother CancerAugust 3, 2023Ya-Xian Wu, Shan Xing, Yu Wang, Bo-Yu Tian, Meng Wu, Xue-Ping Wang, Qi Huang, Xia He, Shu-Lin Chen, Xiao-Hui Li, et al.RE0082; RE0083). The HiScribe T7 High Yield RNA Synthesis Kit was purchased from New England Biolabs (E2040S). The v2 reporter was purchased from Thermo Scientific (Massachusetts, USA; 4479769). Streptavidin-coated

(17) IFI16 phase separation via multi-phosphorylation drives innate immune signalingNucleic Acids ResearchJuly 21, 2023Dawei Liu, Krystal K Lum, Nicholas Treen, Corazón T Núñez, Jinhang Yang, Timothy R Howard, Michael Levine, Ileana M CristeaAlexa Fluor™ 488 Protein Labeling Kit,Thermo Fisher Scientific,A10235 HiScribe® T7 High Yield RNA Synthesis Kit,New England BioLabs,E2040S Applied Biosystems PowerUp SYBR Green Master Mix,Thermo Fisher Scientific,A25778 Oligonucleotides,Oligonucleotides,Oligonucleotides Cy5-VACV70mer

(18) Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiencyNucleic Acids ResearchJuly 21, 2023Karthikeyan Ponnienselvan, Pengpeng Liu, Thomas Nyalile, Sarah Oikemus, Stacy A Maitland, Nathan D Lawson, Jeremy Luban, Scot A Wolfetranscribed from 500 ng purified linearized template using the HiScribe T7 High-Yield RNA Synthesis Kit (NEB, E2040S) with co-transcriptional capping by CleanCap AG (TriLink Biotechnologies, N-7413-5) and full replacement

(19) Human gain-of-function variants in HNF1A confer protection from diabetes but independently increase hepatic secretion of atherogenic lipoproteinsCell GenomicsJuly 12, 2023Natalie DeForest, Babu Kavitha, Siqi Hu, Roi Isaac, Lynne Krohn, Minxian Wang, Xiaomi Du, Camila De Arruda Saldanha, Jenny Gylys, Edoardo Merli, et al.were phenol/chloroform-extracted and in vitro-transcribed with T7 RNA Polymerase HiScribe Mix (NEB E2040S), CleanCap Reagent AG (Tri-Link N-7113) and 5-methoxyuridine triphosphate instead of UTP for 2 h at

(20) RAD21 is the core subunit of the cohesin complex involved in directing genome organizationGenome BiologyJune 28, 2023Yuao Sun, Xin Xu, Wenxue Zhao, Yu Zhang, Keyang Chen, Yongzheng Li, Xiaotian Wang, Mengling Zhang, Boxin Xue, Wanting Yu, et al.then converted into RNA via in vitro transcription (HiScribe™ T7 High Yield RNA Synthesis Kit NEB, #E2040S). RNA templates were converted back to DNA via reverse transcription (MAN0012047 TS Maxima H Minus Reverse

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(1)

7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
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4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

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