BCA Protein Assay Kit 7780S from Cell Signaling Technology

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ItemBCA Protein Assay Kit
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7780S
Quantity1 Kit
Research AreaRelated Products
Detection TargetBCA Protein Assay Kit
ApplicationsFUNC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Hawthorn leaves flavonoids attenuate cardiac remodeling induced by simulated microgravityPharm BiolDecember 1, 2023Liu T, Ma Y, Zhao H, Wang P, Niu Y, Hu Y, Shen X, Zhang M, Yan B, Yu J.was centrifuged at 12000 g for 20 min at 4 °C. Protein concentration was determined using a BCA kit (7780S, Cell Signalling Technology, MA, USA) and adjusted with loading buffer (P0015A, Beyotime, Shanghai,

(2) Pooled endogenous protein tagging and recruitment for scalable discovery of effectors for induced proximity therapeuticsResearch SquareOctober 18, 2023Yevgeniy V. Serebrenik, Yevgeniy Serebrenik, Timothé Maujean, George M. Maujean, George Burslem(MilliporeSigma, P8340). Samples were normalized by bicinchoninic acid (BCA) assay (Cell Signaling, 7780), and loaded on a precast SDS-PAGE gel (Bio-Rad, 4561086). Western blotting followed using standard

(3) Human Placental LRP5 and Sclerostin are Increased in Gestational Diabetes Mellitus PregnanciesThe Journal of clinical endocrinology and metabolismSeptember 18, 2023Papadopoulou A, Thymara E, Maratou E, Kanellopoulos G, Papaevangelou V, Kalantaridou S, Kanellakis S, Triantafyllidou P, Valsamakis G, Mastorakos G.Cell Signaling USA). The concentration of the proteins was determined using the BCA Protein Assay Kit (#7780S, Cell Signaling, USA). Then, Western blotting for expression levels of the LRP5 and of β-actin, used

(4) mTOR Regulation of N-Myc Downstream Regulated 1 (NDRG1) Phosphorylation in Clear Cell Renal Cell CarcinomaInternational Journal of Molecular SciencesMay 27, 2023Anisha Valluri, Jessica Wellman, Chelsea L. McCallister, Kathleen C. Brown, Logan Lawrence, Rebecca Russell, James Jensen, James Denvir, Monica A. Valentovic, Krista L. Denning, et al.Protein concentration in lysate was measured with a BCA protein assay kit (Cell Signaling Technology, 7780). Protein lysate (5 to 15 μg) was diluted in 2x laemmli sample buffer with β-mercaptoethanol, heat denatured

(5) METTL14 Regulates PLAGL2/β-Catenin Signaling Axis to Promote the Development of Nonsmall Cell Lung CancerJournal of oncologyJanuary 1, 2023Zhou Q, Lai X, Gao Y, Chen Q, Xu Y, Liu Y.total protein from cells and tissues. Proteins were quantified according to the BCA protein assay kit (#7780, Cell Signaling Technology, USA) and isolated by 10% SDS-PAGE electrophoresis. Electrotransfer transferred

(6) The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunityiScienceJuly 15, 2022Feng Chen, Runliu Wu, Jiao Liu, Rui Kang, Jinbao Li, Daolin Tangal., 2010). Concentrations of proteins in the supernatant were determined by a BCA Protein Assay Kit (7780, Cell Signaling Technology). Prior to immunoprecipitation, samples containing equal amounts of proteins

(7) Pristimerin inhibits neuronal inflammation and protects cognitive function in mice with sepsis-induced brain injuries by regulating PI3K/Akt signallingPharm BiolDecember 1, 2021Xue W, Li Y, Zhang M.China); and PI3K (4292, Cell Signaling Technology, Shanghai, China). A bicinchoninic acid assay kit (7780, Cell Signaling Technology, Shanghai, China) was used to measure protein concentrations prior to gel electrophoresis

(8) Melatonin inhibits proliferation, migration, and invasion by inducing ROS-mediated apoptosis via suppression of the PI3K/Akt/mTOR signaling pathway in gallbladder cancer cellsAging (Albany NY)September 30, 2021Kunlun Chen, Pengfei Zhu, Wenhui Chen, Kai Luo, Xiao-Jing Shi, Wenlong Zhaidenaturation at 100°C for 10 min, concentrations of proteins were assessed with the BCA Protein Assay Kit (#7780, Cell Signaling Technology Inc, CST, MA, USA) as described [21]. The equal total protein (30 μg) amounts

(9) In vivo inducible reverse genetics in patients' tumors to identify individual therapeutic targetsNature communicationsSeptember 27, 2021Carlet M, Völse K, Vergalli J, Becker M, Herold T, Arner A, Senft D, Jurinovic V, Liu WH, Gao Y, et al.Cell Signaling Technology, Boston, USA) on ice for 30 min and protein concentration measured by BCA assay (#7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple).

(10) A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient modelsScientific reportsJune 16, 2021Liu WH, Völse K, Senft D, Jeremias I.Boston, USA) and PMSF (1:200) on ice for 30 min. Protein concentration was measured by BCA assay (#7780, New England Biolabs, Beverly, USA) and abundance of specific proteins determined by capillary immunoassay.

(11) MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7Folia NeuropathologicaMay 10, 2021Xiaojie Li, Lihe Yuan, Jin Wang, Zun Zhang, Shaojing Fu, Shaobin Wang, Xinhui LiDanvers, MA, USA). Protein concentration was determined with a Bicinchoninic protein assay (BCA) kit (7780S, Cell Signaling Technology, Danvers, MA, USA). All the protein samples were then electrophoresed via

(12) Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6-p-STAT3-p63-JAG2 pathwayStem cell research & therapyMarch 29, 2021Peng W, Chang M, Wu Y, Zhu W, Tong L, Zhang G, Wang Q, Liu J, Zhu X, Cheng T, et al.supernatants were used to measure total protein levels with the Bicinchoninic Acid (BCA) Protein Assay Kit (7780, Cell Signaling Technology [CST], Boston, MA, USA). Myeloperoxidase (MPO) activity determination The

(13) Inhibition of the mevalonate pathway improves myocardial fibrosisExperimental and Therapeutic MedicineJanuary 18, 2021Huifeng Xu, Yi Shen, Chenyu Liang, Haifeng Wang, Junling Huang, Pengcheng Xue, Ming Luoprotein extraction and protein concentration was determined using bicinchoninic acid protein assay reagent (cat. no. 7780; Cell Signaling EXPERIMENTAL AND THERAPEUTIC MEDICINE 21: 224, 2021 3 Table I. Primer sequences

(14) Activity of the growth hormone-releasing hormone antagonist MIA602 and its underlying mechanisms of action in sarcoidosis-like granulomaClinical & translational immunologyJanuary 1, 2021Zhang C, Tian R, Dreifus EM, Hashemi Shahraki A, Holt G, Cai R, Griswold A, Bejarano P, Jackson R, V Schally A, et al.supernatant was collected. Protein concentration was determined by the BCA protein assay kit (Cat #7780; Cell Signaling Technology). Thirty micrograms of total protein was mixed in a reducing sample buffer

(15) GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR SignalingInternational journal of chronic obstructive pulmonary diseaseJanuary 1, 2021Peng W, Wu Y, Zhang G, Zhu W, Chang M, Rouzi A, Jiang W, Tong L, Wang Q, Liu J, et al.Waltham, MA, USA). Total protein levels in BALF were measured by Bicinchoninic Acid Protein Assay Kit (#7780, Cell Signaling Technology, Boston, MA, USA) as per the manufacturer’s protocol. IL-6, IL-1β, and PLAU

(16) Long Noncoding RNA CTC Inhibits Proliferation and Invasion by Targeting miR-146 to Regulate KIT in Papillary Thyroid CarcinomaScientific ReportsDecember 1, 2020Baochun Liao, Shi Liu, Jiafeng Liu, Pulusu Ajay Kumar Reddy, Yong Ying, Yang Xie, Jianhua Wang, Xiangtai ZengTechnology, 9800). Protein concentrations were quantified using BCA assays (Cell Signaling Technology, 7780). Cell lysates (40 μg) were electrophoresed using 12% SDS-PAGE, and were then transferred to a nitrocellulose

(17) Aberrant methylation of GADD45A is associated with decreased radiosensitivity in cervical cancer through the PI3K/AKT signaling pathwayOncology LettersNovember 3, 2020Meng Lou, Rong Li, Ting-Yuan Lang, Li-Ying Zhang, Qi Zhou, Li Lisubsequent experimentation. Protein concentrations were determined using a BCA Protein assay kit (cat. no. 7780, Cell Signaling Technology, Inc.) and ~40 µg protein/lane was separated via SDS‑PAGE on a 10% gel at

(18) Extracellular ubiquitin promotes hepatoma metastasis by mediating M2 macrophage polarization via the activation of the CXCR4/ERK signaling pathwayAnnals of Translational MedicineSeptember 21, 2020Jiajing Cai et al.(Beverly, MA, USA). MMP-9 (#76003), iNOS (#178945), TGF-β (#179695), CXCR4 (#181020/124824) and Ub (#7780) were obtained from Abcam (Cambridge, MA, USA). Animals Male C57BL/6 mice aged 6 to 8 weeks were purchased

(19) miR-125a-5p inhibits glycolysis by targeting hexokinase-II to improve pulmonary arterial hypertensionAging (Albany NY)May 19, 2020Li Luo, Lusheng Xiao, Guili Lian, Huajun Wang, Liangdi Xie

(20) Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-cateninStem cell research & therapyMay 19, 2020Yang X, Ma X, Don O, Song Y, Chen X, Liu J, Qu J, Feng Y.from differently treated MSCs. The protein concentration was determined using the BCA assay (CST, #7780, USA). Proteins were separated on SDS-PAGE gel ahead of being transferred on PVDF membranes (Life Technologies,

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BCA Protein Assay Kit

BCA Protein Assay Kit from Cell Signaling Technology

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BCA Protein Assay Kit from Cell Signaling Technology

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