Description
The Human Angiogenin ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human Angiogenin antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm