ELISA Coating Buffer (5X) from BioLegend

Product Specs
ItemELISA Coating Buffer (5X)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421701
ApplicationsELISA
Quantity30 mL
Format5X concentrated carbonate buffer, pH 9.5
TypeSOLUTION

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.IFNγ capture antibody (diluted 1:500, 507802, BioLegend), respectively, was diluted in coating buffer (421701, BioLegend) and 100 μL per well was distributed in a 96-well plate (423501, Biolegend). After 18 h of

(2) Tumor and local lymphoid tissue interaction determines prognosis in high-grade serous ovarian cancerCell Reports MedicineJuly 18, 2023Haonan Lu, Hantao Lou, Georg Wengert, Reema Paudel, Naina Patel, Saral Desai, Bill Crum, Kristofer Linton-Reid, Mitchell Chen, Dongyang Li, et al.MAB11012), or 0.8ug/mL anti-human GZMB antibody (R&D, DY2906-05) diluted in coating buffer (Biolegend, 421701). The coated plate was then blocked with 1X Assay Diluent A (Biolegend) and washed with 0.05% PBST.

(3) Human Metapneumovirus Reinfection in Aged Mice Recapitulates Increased Disease Severity in Elderly Humans Infected with Human MetapneumovirusImmunoHorizonsJune 1, 2023Olivia B. Parks, Taylor Eddens, Yu Zhang, Tim D. Oury, Anita McElroy, John V. Williamsserved as control. IgG HMPV ELISA A total of 1 μg of TN/94-49 HMPV in 1× ELISA coating buffer (Cat# 421701; BioLegend) was plated per well overnight at 4°C. The remainder of the ELISA was performed as in Alvarez

(4) Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8(+) T cellsNature immunologyMay 1, 2023Corria-Osorio J, Carmona SJ, Stefanidis E, Andreatta M, Ortiz-Miranda Y, Muller T, Rota IA, Crespo I, Seijo B, Castro W, et al.homemade-ELISA: coating antibody: anti-mouse PD-1 (R&D, AF1021, final concentration of 2 µg ml−1; BioLegend, 421701). Detection antibody: anti-hIgG4-HRP (clone HP6023, Abcam; 1:2,000 dilution). IL-2v homemade ELISA:

(5) Alleviation of arthritis through prevention of neutrophil extracellular traps by an orally available inhibitor of protein arginine deiminase 4Scientific ReportsFebruary 23, 2023Chandru Gajendran, Shoichi Fukui, Naveen M. Sadhu, Mohammed Zainuddin, Sridharan Rajagopal, Ramachandraiah Gosu, Sarah Gutch, Saeko Fukui, Casey E. Sheehy, Long Chu, et al.chicken type II collagen (catalog number: 20012, Chondrex) in ELISA coating buffer (catalog number: 421701, BioLegend, Inc., San Diego, CA) overnight at 4 °C. After washing with PBS plus 0.05% Tween-20, the

(6) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.Medical Center, Leiden, The Netherlands",https://ccb.lumc.nl/ ELISA Coating buffer(x5),Biolegend,Cat#421701 ELISA Wash Buffer (x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop

(7) Antibody profiling of environmental protein toxins and virulence factors reveals the prevalence, persistence, and genetics of antibody responsesImmunityJune 1, 2022Julia W. Angkeow, Daniel R. Monaco, Athena Chen, Thiagarajan Venkataraman, Sahana Jayaraman, Cristian Valencia, Brandon M. Sie, Thomas Liechti, Payam Noroozi Farhadi, Gabriela Funez-dePagnier, et al.S7653 Tween-20,Sigma-Aldrich,Cat. # P1379 NP-40,Sigma-Aldrich,Cat. # 492016 ELISA Coating Buffer (5X),BioLegend,Cat. # 421701 Pierce Streptavidin Magnetic Beads,ThermoFisher,Cat. # 88817 QuickPlex 96-Well Plate Plate Pack,Meso

(8) Production and purification of infectious diseases-associated tetramer for staining of CD8+ T cells from PBMCsSTAR ProtocolsMarch 14, 2022Jiezhou Ye, Wen Lei, ChanChan Xiao, Xiangmeng Su, Jie Gao, Xinran Li, Wenxin Li, Pengcheng Wang, Zhenyou Jiang, Guobing ChenBioTECH,Cat#KGR0084 ELISA Wash Buffer 20× concentrate,BioLegend,Cat#421601 ELISA Coating Buffer (5×),BioLegend,Cat#421701 Glutathione oxidized,Sangon biotech,Cat#A610228-0001 Glutathione reduced,Sangon biotech,Cat#A600229-0005 High

(9) Humoral Immunogenicity and Reactogenicity of the Standard ChAdOx1 nCoV-19 Vaccination in TaiwanVaccinesFebruary 17, 2022Chang JH, Chiou JF, Hung CS, Liu MC, Chang HW, Hong SY, Wang CY, Lin YL, Hsieh YC, Chung CL, et al.(10500-CV, 10549-CV, R&D Systems, Minneapolis, MN, USA) in PBS and 50 μL 2× coating buffer (Cat. No. 421701, Biolegend, San Diego, CA, USA) and left overnight at 4 °C. The next day, the plates were washed twice

(10) A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and MechanismsCancers (Basel)February 3, 2022Anissa Nofita Sari et al.(ab46154) (Abcam, Cambridge, UK) was diluted to a final concentration of 4–8 μg/mL in ELISA Coating buffer (421701) (BioLegend Inc., BioLegend Way, San Diego, CA, USA) and incubated overnight at 4 °C in ELISA plates

(11) A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodiesNature CommunicationsDecember 1, 2021Xun Chen, Matteo Gentili, Nir Hacohen, Aviv Regev423501) were coated with 1 µg/ml anti-Flag antibody (Sigma Aldrich, F1804) in coating buffer (BioLegend, 421701) at 4 °C overnight. Plates were washed once with PBST (PBS, ThermoFisher Scientific, with 0.02% TritonX-100),

(12) Handheld Microfluidic Filtration Platform Enables Rapid, Low‐Cost, and Robust Self‐Testing of SARS‐CoV‐2 VirusSmallDecember 1, 2021Jiang Xu, Wenhao Suo, Youlian Goulev, Lei Sun, Liam Kerr, Johan Paulsson, Yan Zhang, Taotao Lao(Zeptometrix, Franklin, MA, USA), in a concentration of 1M /mL in coating buffer (BioLegend, Cat. No. 421701) were transferred and coated to each well of a high affinity, protein-binding ELISA 96-well plate at

(13) A genotype-phenotype screening system using conditionally immortalized immature dendritic cellsSTAR protocolsSeptember 17, 2021Zhao L, Liu P, Xie W, Zhang S, Thieme S, Zitvogel L, Kroemer G, Kepp O.TBS buffer,EUROMEDEX,CAT# ET220-B Tween® 20,Sigma,CAT# P1379 ELISA Coating Buffer (5×),BioLegend,CAT# 421701 HRP-Avidin,BioLegend,CAT# 405103 1-Step™ Ultra TMB-ELISA Substrate Solution,Life Technologies,CAT#

(14) HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responsesCell host & microbeMarch 10, 2021Clayton KL, Mylvaganam G, Villasmil-Ocando A, Stuart H, Maus MV, Rashidian M, Ploegh HL, Walker BD.,R&D Systems,Cat#9269-CD-050 Recombinant HIV-JRCSF gp140 Trimer-Foldon,This paper,NA ELISA Coating Buffer,Biolegend,Cat#421701 "Pierce 16% Formaldehyde, Methanol-Free",ThermoFisher,Cat#28906 Triton X-100,Sigma-Aldrich,Cat#234729 Bovine Serum Albumin

(15) Hepatotoxicity prevention in Acetaminophen-induced HepG2 cells by red betel (Piper crocatum Ruiz and Pav) extract from Indonesia via antioxidant, anti-inflammatory, and anti-necroticHeliyonJanuary 4, 2021Chrismis Novalinda Ginting, I Nyoman Ehrich Lister, Ermi Girsang, Wahyu Widowati, Dewani Tediana Yusepany, Alya Mardhotillah Azizah, Hanna Sari Widya Kusuma2012; Widowati et al., 2019a). 2.4 TNF-α assay This assay was measured using ELISA assay (BioLegend, 421701) and done according to the manufacturer's kit manual. Based on the manual, wavelength at 450 nm was

(16) A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodiesbioRxiv : the preprint server for biologyOctober 30, 2020Chen X, Gentili M, Hacohen N, Regev A.423501) were coated with 1μg/ml anti-Flag antibody (Sigma Aldrich, F1804) in coating buffer (BioLegend, 421701) at 4°C overnight. Plates were washed once with PBST (PBS, ThermoFisher Scientific, with 0.02% TritonX-100),

(17) Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysisScientific reportsSeptember 10, 2020Lim J.setup, 400 ng of anti-human β2m (BioLegend, cat no. 316302) in ELISA coating buffer (BioLegend, cat no. 421701) was coated onto Nunc Maxisorp ELISA plates (BioLegend, cat no. 423501) at 4 °C overnight. The next

(18) Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol DerivativesInternational journal of molecular sciencesJuly 30, 2020Malik V, Garg S, Afzal S, Dhanjal JK, Yun CO, Kaul SC, Sundar D, Wadhwa R.polyclonal antibody (100 ng/mL) in 100 µL coating and diluent buffers (DB) (BioLegend, San Diego, CA, USA, 421701 and 421203, respectively) at room temperature overnight. Wells were washed with 200 µL 0.05% Tween 20

(19) Neuronal Mitochondria Modulation of LPS-Induced NeuroinflammationThe Journal of neuroscience : the official journal of the Society for NeuroscienceFebruary 19, 2020Harland M, Torres S, Liu J, Wang X.Optical 96-well plates were coated with polyclonal antigen capture antibody in ELISA Coating Buffer (421701, BioLegend) or, for CX3CL1 direct ELISA, 20 ␮g/ml mouse brain extract in PBS overnight at 4°C. Wells

(20) Biological Responses of Onion-Shaped Carbon NanoparticlesNanomaterials (Basel)July 15, 2019Jaehee Jang, Youngjun Kim, Jangsun Hwang, Yonghyun Choi, Masayoshi Tanaka, Eunah Kang, Jonghoon Choiunlabeled capture antibody was diluted to a final concentration of 0.5–8 µg/mL in coating buffer (Cat. No. 421701, BioLegend, CA USA) and 100 µL were transferred to each well of a high-affinity, protein-binding ELISA

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ELISA Coating Buffer (5X) from BioLegend

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ELISA Coating Buffer (5X) from BioLegend

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