Fig 1: HSP mRNA expression (hsp70.1, hspa1a, and hspa1b) is not dependent on MCA occlusion time (Kruskal-Wallis tests, p>0.05). Data are expressed as the mean±SD. No significant differences in HSP mRNA expression (hsp70.1, hspa1a, hspa1b) in the bilateral (Rt : contralateral, Lt : ipsilateral) hippocampi of mice are evident (Kruskal-Wallis tests, p>0.05). Data are expressed as the mean±SD. HSP : heat shock protein, mRNA : messenger RNA, MCA : middle cerebral artery, SD : standard devicetion.
Fig 2: Effect of Au NPs@polyphenols on the Ca2+ level in SH-SY5Y (a) and MCF-7 (b) cells after 24 h and 48 h of exposure to two doses (c1 and c2) of Au NPs@polyphenols at 37 °C and 43 °C, following the procedure described in the section, Materials. The data are reported as the mean ± SD from three independent experiments; * p < 0.05, compared with control (n = 8). (c,d) The effect of Au NPs@polyphenols on the ROS level in SH-SY5Y and MCF-7 cells after 24 h and 48 h of exposure to two doses (c1 and c2) of Au NPs@polyphenols at 37 °C and 43 °C, following the procedure described in the section, Materials. The ROS generation of NP-treated cells is expressed relative to non-treated control cells. As a positive control (P), the cells were incubated with 500 µM of H2O2, showing a ca. 300% DCFH-DA increase (not shown). The data are reported as the mean ± SD from three independent experimens; * p < 0.05, compared with the control (n = 8). (e–l) Representative fluorescence images of SH-SY-5Y and MCF-7 cells exposed to the c2 of Au NPs@polyphenols at 37 °C and 43 °C, following the procedure described in the section, Materials. (m) ELISA quantitative analyses of HSP70 in SH-SY5Y and MCF-7 cells treated with Au NPs@polyphenols at 37 °C and 43 °C at the c2 concentration, following the procedure described in the section, Materials. Histogram showing the levels of HSP70 expressed in ng/mL in SH-SY5Y (n) and MCF-7. The data are presented as the mean ± standard deviation of three independent experiments: * p < 0.05.
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