Description
Principle of the assay: human TIE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIE2 has been precoated onto 96-well plates. Standards(NSO, A23-K745) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIE2 amount of sample captured in plate.
Background: Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion.i Angiogenesis is coordinated with follicular cell growth in goitrogenesis.The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis.ii Tie2/Ang-1signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche.iii And theTie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.iv