Human Interleukin 10,IL-10 ELISA Kit CSB-E04593h from CUSABIO Technology LLC

Product Specs
ItemHuman Interleukin 10,IL-10 ELISA Kit
CompanyCUSABIO Technology LLC
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberCSB-E04593h
Quantity96T
Detection Range12.5 pg/mL-800 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, urine, cell culture supernates, ascitic fluid, cerebrospinal fluid (CSF), saliva
Sensitivity3.12 pg/mL
Volume50-100ul
Detection Targetinterleukin 10
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
ReactivityHomo sapiens (Human)
NCBI Full Gene Nameinterleukin 10
NCBI Gene AliasesCSIF, GVHDS, IL-10, IL10A, TGIF
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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CUSABIO Technology LLC

7707 Fannin St.

Ste 200-V126

Houston, TX 77054

United States

Phone: 301-363-4651

Email: support@cusabio.com

Company Profile

Website: www.cusabio.com

(1) Circulating cytokine profile and modulation of regulatory T cells in chronic hepatitis B patients with type 2 diabetes mellitusBosnian Journal of Basic Medical SciencesFebruary 1, 2023Wang He, Shu Hao, Xiaohui Dong, Dandan Zhang, Zhen Jiacommercial enzyme-linked immunosorbent assay (ELISA) kits (CUSABIO, Wuhan, Hubei Province, China; Catalog# CSB-E04593h and CSB-E13126h) following manufacturer’s instructions. mRNA expression detection in Tregs mRNA expression

(2) Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent PreeclampsiaCell transplantationJanuary 1, 2023Liu H, Yu L, Ding Y, Peng M, Deng Y.pg/mL,CUSABIO (China) Rat IL-4,CSB-E04635r,15.6 pg/mL-1000 pg/mL,3.9 pg/mL,CUSABIO (China) Human IL-10,CSB-E04593h,31.25 pg/mL-2000 pg/mL,7.8 pg/mL,CUSABIO (China) Rat IL-10,CSB-E04595r,62.5 pg/mL-4000 pg/mL,15.6 pg/mL,CUSABIO

(3) Versatile-in-All-Trades: Multifunctional Boron-Doped Calcium-Deficient Hydroxyapatite Directs Immunomodulation and RegenerationACS Biomaterials Science & EngineeringJuly 11, 2022Ahmet Engin Pazarçeviren, Sema Akbaba, Zafer Evis, Ayşen Tezcanerkit (E4588, Biovision, USA). At the same time, aliquots were tested using a human IL-10 ELISA kit (CSB-E04593h, Cusabio, China). Afterward, media were discarded, cells were rinsed with PBS twice, and MTT reagent

(4) Luteolin activates M2 macrophages and suppresses M1 macrophages by upregulation of hsa_circ_0001326 in THP-1 derived macrophagesBioengineeredMarch 1, 2022Gong B, Zheng Y, Li J, Lei H, Liu K, Tang J, Peng Y.TNF-α (Cusabio, Wuhan, China; cat: CSB-E04740h), IL-6 (Cusabio, cat: CSB-E04638h), IL-10 (Cusabio, cat: CSB-E04593h), and IL-RA (Cusabio, cat: CSB-E04629h). Bioinformatics analysis To explore the potential mechanism

(5) Systemic low-grade inflammation and depressive symptomology at chronic phase of ischemic stroke: The chain mediating role of fibrinogen and neutrophil countsBrain, behavior, and immunityFebruary 1, 2022Sun W, Miao J, Song Y, Wang Y, Pan C, Li G, Zhao X, Lan Y, Qiu X, Zhu S, et al.

(6) Elevated DLL3 in stomach cancer by tumor-associated macrophages enhances cancer-cell proliferation and cytokine secretion of macrophagesGastroenterology ReportJanuary 25, 2022Jian-Bin Ye, Jun-Jie Wen, Dan-Lin Wu, Bing-Xin Hu, Mei-Qun Luo, Yan-Qing Lin, Yun-Shan Ning, Yan Li(ELISA), including IL-1β (CSB-E08053h; CUSABIO; Wuhan, Hubei, China), IL-12 (CSB-E04599h; CUSABIO), IL-10 (CSB-E04593h; CUSABIO), and IL-33 (CSB-E13000h; CUSABIO). The results were quantified using a microplate reader (EPOCH2;

(7) Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male RatsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2022Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y.CSB-E04638h/CSB-E04639m), TNF-α ELISA Kit (Cusabio, CSB-E04740h/CSB-E04639m), IL-10 ELISA Kit (Cusabio, CSB-E04593h/CSB-E04594m), and IL-4 ELISA Kit (Cusabio, CSB-E04633h/CSB-E04634m) to detect the levels of NPY, T,

(8) The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart FailureMediators of InflammationOctober 27, 2021Zhenhua Wang, Zhaoling Cai, Markus W. Ferrari, Yilong Liu, Chengyi Li, Tianzhang Zhang, Guorong Lyulevel of IL-6 (CSB-E04638h, Cusabio biotech, China), IL-8 (CSB-E04641h, Cusabio biotech, China), IL-10 (CSB-E04593h, Cusabio biotech, China), and TNF-α (CSB-E04740h, Cusabio biotech, China) in the blood samples was determined

(9) Adipose-derived stem cells promote diabetic wound healing via the recruitment and differentiation of endothelial progenitor cells into endothelial cells mediated by the VEGF-PLCγ-ERK pathwayArchives of biochemistry and biophysicsOctober 15, 2020Chen L, Zheng Q, Liu Y, Li L, Chen X, Wang L, Wang L.

(10) Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitisMolecular immunologySeptember 1, 2020Li J, Lin X, Liu X, Ma Z, Li Y.

(11) High lymphocyte‑to‑monocyte ratio is associated with low α‑fetoprotein expression in patients with hepatitis B virus‑associated hepatocellular carcinomaMolecular Medicine ReportsJuly 29, 2020Haixia Wang, Yu Xiang, Xinyu Li, Shuang Liu, Linxiu Liuand the expression levels of IL-10 were assessed using an ELISA kit (Human IL-10 ELISA kit; cat. no. CSB-E04593h; Cusabio Biotech Co., Ltd.) and MB-530 microplate reader to determine the cut-off value. Each group

(12) In major depression, increased kappa and mu opioid receptor levels are associated with immune activationActa neuropsychiatricaApril 1, 2020Al-Hakeim HK, Zeki Al-Fadhel S, Al-Dujaili AH, Maes M.

(13) MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathwayEBioMedicineFebruary 1, 2020Jiang Y, Zhou J, Zhao J, Hou D, Zhang H, Li L, Zou D, Hu J, Zhang Y, Jing Z.

(14) Rhein Suppresses Neuroinflammation via Multiple Signaling Pathways in LPS-Stimulated BV2 Microglia CellsEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Zheng P, Tian X, Zhang W, Yang Z, Zhou J, Zheng J, Cui H, Tang T, Luo J, Wang Y.

(15) Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathwayTheranosticsJanuary 1, 2020Ma F, Hao H, Gao X, Cai Y, Zhou J, Liang P, Lv J, He Q, Shi C, Hu D, et al.Enzyme-linked immunosorbent assay (ELISA) for cytokines and melatonin The concentrations of cytokines, including IL-10 (Cat #CSB-E04593h), IL-17 (Cat #CSB-E12819h), IL-22 (Cat #CSB-E13418h), and TGF-β (Cat #CSB-E04725h), were measured in

(16) Development of a Novel Neuro-immune and Opioid-Associated Fingerprint with a Cross-Validated Ability to Identify and Authenticate Unknown Patients with Major Depression: Far Beyond Differentiation, Discrimination, and ClassificationMolecular neurobiologyNovember 1, 2019Al-Hakeim HK, Al-Fadhel SZ, Al-Dujaili AH, Carvalho A, Sriswasdi S, Maes M.

(17) Si-ni-tang (a Chinese herbal formula) for improving immunofunction in sepsis: study protocol for a pilot randomized controlled trialTrialsAugust 28, 2019Zeng R, Zheng Y, Fan R, Zhou G, Zhang Y, Mai S, Xie D, Weng Y, Du J, Han Y, et al.with an additional 2–3 mL of peripheral venous blood using ELISA kits (Cusabio, CSB-E04638h; Cusabio, CSB-E04593h; Cusabio, CSB-E04740h). The serum will be separated by centrifugation at 3000 rpm for 20 min and stored

(18) Shikonin Controls the Differentiation of CD4⁺CD25⁺ Regulatory T Cells by Inhibiting AKT/mTOR PathwayInflammationAugust 1, 2019Zhang X, Li J, Yu Y, Lian P, Gao X, Xu Y, Geng L.

(19) Interleukin-35 modulates the balance between viral specific CD4⁺CD25⁺CD127^dim/- regulatory T cells and T helper 17 cells in chronic hepatitis B virus infectionVirology journalApril 16, 2019Yang L, Jia S, Shao X, Liu S, Zhang Q, Song J, Wang W, Jin Z.

(20) Six-month clinical outcomes of non-surgical periodontal treatment with antibiotics on apoptosis markers in aggressive periodontitisOral diseasesApril 1, 2019Aral K, Aral CA, Kapila Y.

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Human Interleukin 10,IL-10 ELISA Kit from CUSABIO Technology LLC

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Human Interleukin 10,IL-10 ELISA Kit from CUSABIO Technology LLC

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