Human/Rat beta-Amyloid (42) ELISA Kit, High Sensitivity 292-64501 from FUJIFILM Wako Chemicals U.S.A. Corp

Product Specs
ItemHuman/Rat beta-Amyloid (42) ELISA Kit, High Sensitivity
CompanyFUJIFILM Wako Chemicals U.S.A. Corp
Price Supplier Page
Catalog Number292-64501
ReactivityHuman, Mouse, Rat
Detection TargetHuman/Rat β-Amyloid (42)
SpeciesHuman/Rat
Quantity96 Tests
MethodELISA
ApplicationsELISA
Sample TypeTissue homogenate, CSF, Plasma, Serum, Culture medium
Detection Range0.1-20 pM
Assay TimeAbout 20 hours
Sensitivity0.1pM

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FUJIFILM Wako Chemicals U.S.A. Corp

1600 Bellwood Road

Richmond, VA 23237-1326

United States

Phone: 804-271-7677
Fax: 804-271-7791

Email: wkuslabchem@fujifilm.com

Company Profile

Website: www.wakousa.com

(1) Chronic, Mild Hypothermic Environmental Temperature Does Not Ameliorate Cognitive Deficits in an Alzheimer’s Disease MouseThe Journals of Gerontology: Series AJune 1, 2023Samuel McFadden, Lindsey N Sime, Ma Kayla F Cox, Caleigh A Findley, Mackenzie R Peck, Kathleen Quinn, Yimin Fang, Andrzej Bartke, Erin R Hascup, Kevin N HascupAβ 42 concentrations were determined using the Human/Rat β amyloid ELISA kits (WAKO Chemicals; Cat: 292-64501) according to the manufacturer recommended protocols. Amyloid Plaque Staining and Semiquantification A

(2) Activation of the hypoxia response protects mice from amyloid-β accumulationCellular and molecular life sciences : CMLSJuly 19, 2022Ollonen T, Kurkela M, Laitakari A, Sakko S, Koivisto H, Myllyharju J, Tanila H, Serpi R, Koivunen P.concentration of Aβ42 load was determined using Human/Rat Beta Amyloid ELISA Kit (Wako, High-Sensitive, 292-64501) according to the manufacturer’s instructions. The final dilution factors were 1:2000 (4 months) and

(3) CRISPR-Cas9 treatment partially restores amyloid-Î² 42/40 in human fibroblasts with the Alzheimer's disease PSEN 1 M146L mutationMolecular Therapy - Nucleic AcidsJune 14, 2022Evangelos Konstantinidis, Agnieszka Molisak, Florian Perrin, Linn Streubel-Gallasch, Sarah Fayad, Daniel Y. Kim, Karl Petri, Martin J. Aryee, Ximena Aguilar, Bence GyÃ¶rgy, et al.collected and subjected to ELISAs specific for Aβ40 (BNT77-BA27; no. 294-64701) and Aβ42 (BNT77-BC05; no. 292-64501; Wako Pure Chemicals Industries, Osaka, Japan). Both assays were performed according to the manufacturer’s

(4) High Correlation among Brain-Derived Major Protein Levels in Cerebrospinal Fluid: Implication for Amyloid-Beta and Tau Protein Changes in Alzheimer’s DiseaseMetabolitesApril 15, 2022Kyoka Hoshi et al.Kit Wako II (298-64601, FUJIFILM Wako) for Aβ40; Human βAmyloid (42) ELISA Kit Wako High Sensitive (292-64501, FUJIFILM Wako) for Aβ42. 4.4. Statistical Analyses Statistical analyses were performed using SPSS

(5) Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimer’s DiseaseMetabolitesSeptember 10, 2021Kyoka Hoshi et al.Kit Wako II (298-64601, FUJIFILM Wako) for Aβ40; Human βAmyloid (42) ELISA Kit Wako High Sensitive (292-64501, FUJIFILM Wako) for Aβ42. 5.7. Preparation of Tissue Sections and Pairs of Mirror Image Sections Brain

(6) Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotypePLoS biologySeptember 1, 2021Lam V, Takechi R, Hackett MJ, Francis R, Bynevelt M, Celliers LM, Nesbit M, Mamsa S, Arfuso F, Das S, et al.manufacturer’s instruction. Mouse Aβ concentrations were determined with commercial ELISA (Wako 294–62501 and 292–64501), while human Aβ was measured with Simoa Human Neurology 3-plex A (N3PA) according to manufacturer’s

(7) Impairment of visual cortical plasticity by amyloid-beta speciesNeurobiology of DiseaseJuly 1, 2021Christopher M. William, Matthew A. Stern, Xuewei Pei, Lubna Saqran, Margish Ramani, Matthew P. Frosch, Bradley T. HymanELISA kits were used to quantify concentrations of Aβ40 and Aβ42 in homogenates (kits 294–64701 and 292–64501, respectively; Wako). Averages of concentrations per group are reported with standard deviations. 2.3.

(8) Overlapping roles of JIP3 and JIP4 in promoting axonal transport of lysosomes in human iPSC-derived neuronsMolecular Biology of the CellMay 15, 2021Swetha Gowrishankar, Lila Lyons, Nisha Mohd Rafiq, Agnes Roczniak-Ferguson, Pietro De Camilli, Shawn M. FergusonTris-EDTA and then processed for ELISA-based measurements of Aβ42 using a high-sensitivity ELISA kit (Wako; 292–64501). Standard curves were generated using Aβ1-42 peptide standards for each set of experiments. Immunofluorescence analysis

(9) Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activationMolecular Medicine ReportsJanuary 5, 2021Qing-Qing Xie et al.neurons and the hippocampal homogenate of rats were detected using ELISA kits (cat. nos. 294-62501 and 292-64501; Wako Chemicals GmbH), according to the manufacturer's instructions. For human or rat Aβ40 and Aβ42

(10) Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of AβCellsNovember 15, 2020Marttinen M, Ferreira CB, Paldanius KMA, Takalo M, Natunen T, Mäkinen P, Leppänen L, Leinonen V, Tanigaki K, Kang G, et al.2482 5 of 21 40 (294-64701) (Wako, FUJIFILM Wako Chemicals, VA, USA) and β-amyloid 42 (High-Sensitive, 292-64501) ELISA kits (Wako, FUJIFILM Wako Chemicals, VA, USA), according to manufacturer’s protocol. 2.8. Biotinylation

(11) Sleep and diurnal rest-activity rhythm disturbances in a mouse model of Alzheimer’s diseaseSleepNovember 12, 2020Mikolaj J Filon, Eli Wallace, Samantha Wright, Dylan J Douglas, Lauren I Steinberg, Carissa L Verkuilen, Pamela R Westmark, Rama K Maganti, Cara J Westmarkwith Wako Human/Rat Aβ40 (catalog #294–64701) and Wako Human/Rat Aβ 1–42 high sensitivity (catalog #292–64501) ELISA kits per the manufacturer instructions. Plasma samples were diluted fourfold with standard diluent

(12) Vaccination Prevented Short-Term Memory Loss, but Deteriorated Long-Term Spatial Memory in Alzheimer's Disease Mice, Independent of Amyloid-β PathologyJournal of Alzheimer's disease reportsJuly 23, 2020Oberman K, Gouweleeuw L, Hoogerhout P, Eisel ULM, van Riet E, Schoemaker RG.heart samples at sacrifice. In a subset of mice, plasma levels of Aβ1–42 were measured by ELISA (code 292-64501, WAKO, USA), according to manufacturer’s procedures; The kit is constructed as a sandwich ELISA format

(13) AP ‐2 reduces amyloidogenesis by promoting BACE 1 trafficking and degradation in neuronsEMBO reportsJune 4, 2020Sujoy Bera et al.peptides in the media of cultured neurons were analyzed at DIV 11–12 using WAKO kit (# 294‐64701 and # 292‐64501, respectively) following the manufacturer's instructions. Immunohistochemistry Mice were sacrificed

(14) Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening systemFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJune 1, 2020Chiu YW, Hori Y, Ebinuma I, Sato H, Hara N, Ikeuchi T, Tomita T.ELISA Kit (294-64701, WAKO, Osaka, Japan) and Human/Rat β -Amyloid (42) ELISA Kit, High Sensitivity (292-64501, WAKO, Osaka, Japan), as described (26). Aβ levels were then standardized by protein concentrations

(15) Early sirtuin 2 inhibition prevents age-related cognitive decline in a senescence-accelerated mouse modelNeuropsychopharmacologyJanuary 1, 2020Teresa Diaz-Perdigon, Francisco B. Belloch, Ana Ricobaraza, Elghareeb E. Elboray, Takayoshi Suzuki, Rosa M. Tordera, Elena Puertaas previously described [37]. High-sensitive enzyme-linked immunosorbent assay kits from Wako (cat#292–64501 for Aβ42 and cat#294-64701 for Aβ40, Wako Chemicals, Richmond, VA) were used following the manufacturer’s instructions. Quantiﬁcation

(16) Opposite Roles of δ- and μ-Opioid Receptors in BACE1 Regulation and Alzheimer's InjuryFrontiers in cellular neuroscienceJanuary 1, 2020Xu Y, Zhi F, Balboni G, Yang Y, Xia Y.K388-100, Milpitas, CA, United States). Human/Rat β Amyloid(42) ELISA Kit were obtained from Wako (Cat: 292-64501, Osaka, Japan). Cell Cultures and Cell Treatments An original highly differentiated rat PC-12 cell

(17) Donepezil modulates amyloid precursor protein endocytosis and reduction by up-regulation of SNX33 expression in primary cortical neuronsScientific ReportsDecember 1, 2019Yuki Takada-Takatori, Shota Nakagawa, Riko Kimata, Yousuke Nao, Yumiko Mizukawa, Tetsuro Urushidani, Yasuhiko Izumi, Akinori Akaike, Katsuharu Tsuchida, Toshiaki KumeELISA Kit Wako II (Code #294-64701) or Human/Rat β amyloid (42) ELISA Kit Wako, High-Sensitive (Code #292-64501) purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Microplate reader (Model 680;

(18) Conformational Dynamics of Transmembrane Domain 3 of Presenilin 1 Is Associated with the Trimming Activity of γ-SecretaseThe Journal of NeuroscienceOctober 23, 2019Tetsuo Cai, Kanan Morishima, Shizuka Takagi-Niidome, Aya Tominaga, Taisuke Tomita␤-amyloid (40) ELISA Kit (#294 – 62501, Wako) and human/rat ␤-amyloid (42) ELISA Kit, High Sensitivity (#292– 64501, Wako). Luciferase assay. Luciferase assay for amyloid precursor protein intracellular domain (AICD)

(19) Serine Phosphorylation of IRS1 Correlates with Aβ-Unrelated Memory Deficits and Elevation in Aβ Level Prior to the Onset of Memory Decline in ADNutrientsAugust 17, 2019Wei Wang, Daisuke Tanokashira, Yusuke Fukui, Megumi Maruyama, Chiemi Kuroiwa, Takashi Saito, Takaomi C. Saido, Akiko TaguchiChemical Corp., Osaka, Japan), and the Human/Rat/Mouse β Amyloid (1–42) ELISA Kit Wako, High Sensitivity (#292-64501, Fujifilm Wako Pure Chemical Corp.) in accordance with the manufacturers’ instructions. 2.6. Statistics All

(20) Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer’s disease pathogenesisNature NeuroscienceAugust 1, 2019Pilar Esteve et al.human Aβ42 ELISA Kit 29 876 (Invitrogen, cat: KHB3441), the human-rat Aβ42 ELISA kit (WAKO, cat: 292-64501) and 877 the human sAPPα ELISA kit (MyBioSource, San Diego, CA, USA, cat: MBS915453). 878 Samples

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Human/Rat beta-Amyloid (42) ELISA Kit, High Sensitivity from FUJIFILM Wako Chemicals U.S.A. Corp

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FUJIFILM Wako Chemicals U.S.A. Corp

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Human/Rat beta-Amyloid (42) ELISA Kit, High Sensitivity from FUJIFILM Wako Chemicals U.S.A. Corp

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