Fig 1: Scatter plots of fasting plasma FGF21 levels (A) and the postprandial FGF21 response (B) at baseline with weight change after 12 weeks of intervention on the high-nutrient-quality diet (HQ, dark green) and low-nutrient-quality diet (LQ, light green). Correlations were tested using Spearman correlation coefficients (LQ and HQ group combined in black).
Fig 2: Effects of two 12-week energy restriction diets (HQ, high-nutrient-quality diet; LQ, low-nutrient-quality diet) or control group (CON) on fasting and postprandial plasma FGF21 levels. (A) Geometric mean (95% CI) change in fasting plasma FGF21 upon a 12-week diet, as tested by ANCOVA with adjustment for baseline fasting FGF21 levels. (B) Geometric mean (95% CI) change in postprandial FGF21 response (∆120–0 min) to a mixed meal upon a 12-week diet, as tested by univariate ANOVA.
Fig 3: Postprandial FGF21 response at baseline and correlations between plasma FGF21 and markers of metabolic health. (A) Box plot with individual data points of the change in plasma FGF21 levels from the fasting state to 120 min after the consumption of the mixed meal in the complete population at baseline (p = 0.055, as tested using a paired-samples T-test). The box plot represents the 5th percentile, first quartile, median, third quartile, and 95th percentile. (B) Individual postprandial FGF21 responses according to baseline HOMA-IR: =2.5 or >2.5 (p = 0.012 for the difference between groups, as tested with ANCOVA, adjusted for fasting FGF21, age, gender, and BMI. (C) Spearman correlations between fasting FGF21 and the postprandial FGF21 response with cardiometabolic parameters at baseline (left) and in response to a 12-week intervention (right). Abbreviations: BMI, body mass index; AT, adipose tissue; IHL, intra-hepatic lipid content; HbA1c, glycated hemoglobin A1c; iAUC, incremental area under the curve; HDL, high-density lipoprotein; HOMA-IR, homeostatic model assessment of insulin resistance; ALAT, aminotransferase; ASAT, aspartate aminotransferase; ?GT, gamma-glutamyl transferase.
Fig 4: Fasting plasma FGF21 levels (adjusted geometric means with 95% CI) according to tertiles (T1: lowest tertile, T3: highest tertile) of habitual (macro)nutrient intake as % of daily energy intake (en%) (A–C,E,F), habitual alcohol consumption (D), and sweet-taste preference (G). FGF21 levels were lower in the highest tertile of polysaccharide intake compared to the lower tertiles (overall p = 0.022; tested by ANCOVA with adjustment for gender and LSD post-hoc testing; * p = 0.035; ** p = 0.009) and did not differ between tertiles of other nutrient intakes.
Supplier Page from Abcam for Human FGF-21 ELISA Kit