Description
This cartridge kit contains materials required for preparing Helios® gene gun cartridges. It includes 0.5 g PVP (MW 360,000), 5 desiccant cartridges, 5 cartridge collection/storage vials, and 50' of Tefzel tubing. The Bio-Rad Helios gene gun is a convenient, handheld device that uses biolistic particle delivery to allow rapid and direct transfection of both in vivo and in vitro targets. The Helios gene gun can transfect a wide range of targets - any type of cell that can be made accessible to the Helios gene gun nozzle can be transformed. It uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers directly into target cells. This allows for transfer of large DNA fragments, codelivery of more than one plasmid, and requires only small amounts of DNA.