Fig 2: Position distribution of the SNPs in the S100B gene.

Fig 3: Western blot analysis of the S100B levels in SH-SY5Y cells after transfection of the three different miRNAs. (A) We detected the S100B protein level using western blot analysis. (B) Densitometry analysis to determine the ratio of S100B to GAPDH. All data are the average of three measurements (*P < 0.05).

Fig 4: Verification of the interaction between candidate miRNAs and the 3'-UTR of the S100B gene, which contains the rs9722 loci, using the luciferase assay. (A) The candidate miRNAs that can bind to the rs9722 locus in the 3'-UTR of the S100B gene. The red letters show the rs9722 locus. (B) Plasmids containing different genotypes and artificial mutations. The black frame indicates the rs9722 locus. (C) Detection of miRNA levels in the 293T cells after transfection. (D) Fluorescence intensity after transfection of the miRNAs and the plasmids containing the different rs9722 alleles or mutations (*P < 0.05).