Fig 1: Relative gene expression of cytokines. Fibroblasts from 5 FOP and 5 control cell lines were serum-starved overnight (-FBS) before treatment with Activin A (+Activin A), BMP4 (+BMP4) and TGFß1 (+TGFß) for 6 h. Relative gene expression was measured by qPCR for INHBA, TGFBR1, IL6, and IL1b; YWHAZ was used to normalize gene expression. Data is shown as the expression levels per cell line and their mean. * p < 0.05, ** p < 0.005 as determined by 2-way ANOVA.