TUNEL Assay Kit - FITC ab66108 from Abcam

Product Specs
ItemTUNEL Assay Kit - FITC
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab66108
Quantity50 test
MethodFlow Cyt
Sample TypeAdherent cells, Suspension cells
Assay Time2h 30m
ApplicationsCellular Assay
TypeDirect
Detection TargetDNA fragmentation

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophagesJ Biomed SciDecember 18, 2023Mi-Hyun Lee, Hye Lin Kim, Hyejun Seo, Sangkwon Jung, Bum-Joon Kim× 105) were seeded in 2-well chamber slides (Thermo Scientific, 154461). A TUNEL assay kit (Abcam, ab66108) was used to detect DNA fragmentation and apoptotic cells. Infected BMDMs were fixed with 4% PFA for

(2) Dysregulation of a lncRNA within the TNFRSF10A locus activates cell death pathwaysCell Death DiscoveryJuly 13, 2023Tadeusz J. Kaczynski, Nadine J. Husami, Elizabeth D. Au, Michael H. Farkastransferase dUTP nick end labeling TUNEL staining was carried out using a FITC TUNEL assay kit (Abcam, Cat#: ab66108) according to the manufacturer’s instructions with minor alterations. Briefly, ARPE-19 cells were collected

(3) Post‐inflammatory administration of N‐acetylcysteine reduces inflammation and alters receptor levels in a cellular model of Parkinson's diseaseFASEB BioAdvancesJuly 1, 2023Zeynep Bengisu Kaya, Elif Karakoc, Pamela J. McLean, Esen Saka, Pergin Atillaapoptotic cells. The TUNEL evaluation was carried out using a TUNEL experimental kit (TUNEL Assay Kit‐FITC ab66108; Abcam) according to the manufacturer's instructions. Cells were cultured on 6‐cm petri dishes without

(4) Diplacone Isolated from Paulownia tomentosa Mature Fruit Induces Ferroptosis-Mediated Cell Death through Mitochondrial Ca2+ Influx and Mitochondrial Permeability TransitionInternational Journal of Molecular SciencesApril 11, 2023Eun Sol Oh, Hyung Won Ryu, Yu Na Song, Myung-Ji Kang, Yang Hoon Huh, Ji-Yoon Park, Seon Min Oh, Su-Yeon Lee, Yhun Jung Park, Doo-Young Kim, et al.was purchased from Enzo Life Sciences (Farmingdale, NY, USA). The FITC-conjugated TUNEL Assay Kit (ab66108) and lipid peroxidation (MDA) assay kit (ab118972) were acquired from Abcam. 4.2. Plant Material Sample

(5) The Effects of Glutathione on Clinically Essential Fertility Parameters in a Bleomycin Etoposide Cisplatin Chemotherapy ModelLife (Basel)March 17, 2023Hale Bayram, Yaprak Donmez Cakil, Mustafa Erinc Sitar, Gamze Demirel, Belgin Selam, Mehmet Cincikdeoxynucleotidyl transferase dUTP nick end labeling) method using the Abcam TUNEL assay kit-FITC (CAS: 3326-32-7; ab66108; Cambridge, UK). First, the sperm were fixed in 1% paraformaldehyde for 15 min at −20 °C. The fixed

(6) Progression of Thoracic Aortic Dissection Is Aggravated by the hsa_circ_0007386/miR-1271-5P/IGF1R/AKT Axis via Induction of Arterial Smooth Muscle Cell ApoptosisBiomedicinesFebruary 15, 2023Xinsheng Xie, Xiang Hong, Shichai Hong, Yulong Huang, Gang Chen, Yihui Chen, Yue Lin, Weifeng Lu, Weiguo Fu, Lixin WangTUNEL staining assay was performed according to the method steps provided by the TUNEL Assay Kits (ab66108, Abcam, USA). Western blot assays were performed as previously described [20]. Detailed methods are

(7) Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic ReprogrammingCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Lu T, Li Q, Lin W, Zhao X, Li F, Ji J, Zhang Y, Xu N.apoptotic cells were detected using the in situ BrdU-Red DNA fragmentation (TUNEL) assay kit (Abcam，cat. #ab66108) and counterstained with DAPI (Tocris, cat. #5748). Immunohistochemistry Paraffin liver sections were

(8) Mechanism of paracrine communications between hepatic progenitor cells and endothelial cellsCellular signallingDecember 1, 2022Lee S, Karns R, Shin S.respectively. The BrdU Cell Proliferation Assay Kit (2750, Millipore, MA, USA) and the TUNEL Assay Kit - FITC (ab66108, Abcam, Cambridge, UK) were used per the manufacturer’s manual. 2.6. Tubulogenesis assay The In Vitro Angiogenesis Assay Kit

(9) Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinomaWorld Journal of Surgical OncologyDecember 1, 2022Longhua Feng, Pengjiang Cheng, Zhengyun Feng, Xiaoyu Zhangend labeling (TUNEL) staining Tissue fractions from each group were analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously [24]. Images were captured using a fluorescence microscope

(10) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasisCardiovascular DiabetologyDecember 1, 2022Rongjun Zou, Wanting Shi, Junxiong Qiu, Na Zhou, Na Du, Hao Zhou, Xinxin Chen, Li Maantibodies. Images were acquired using a confocal microscope (LCS-SP8-STED, Leica). The TUNEL assay (Abcam, #ab66108) was conducted based on the manufacturer’s instructions. MtROS levels in HCAECs were measured using

(11) Icariin-loaded hydrogel with concurrent chondrogenesis and anti-inflammatory properties for promoting cartilage regeneration in a large animal modelFrontiers in Cell and Developmental BiologyNovember 24, 2022Songshan Xu, Shaohua Zhao, Yanpeng Jian, Xinwei Shao, Dandan Han, Fan Zhang, Chen Liang, Weijie Liu, Jun Fan, Zhikui Yang, et al.against collagen type II, IL-1β, IL-6, TNF-α, and TUNEL (No. ab188570, ab254360, ab303458, ab183218, and ab66108, Abcam) at 4°C. After rinsing, the samples were treated with goat anti-rabbit secondary antibody (1:1000,

(12) Mitochondrial derived peptide MOTS‐c prevents the development of heart failure under pressure overload conditions in miceJournal of Cellular and Molecular MedicineNovember 1, 2022Peng Zhong, Jianye Peng, Yewen Hu, Jun Zhang, Caijie Shenvascular area. Apoptosis was assessed using the TdT‐mediated dUTP nick end labeling (TUNEL) assay kit (ab66108 from Abcam), and slides were viewed using a fluorescence microscope (Olympus). Percentage of TUNEL‐positive

(13) The ex vivo human translaminar autonomous system to study spaceflight associated neuro-ocular syndrome pathogenesisNPJ microgravityOctober 28, 2022Peng M, Curry SM, Liu Y, Lohawala H, Sharma G, Sharma TP.the sections were stained according to manufacturer’s protocol for the TUNEL assay (catalog number ab66108, Abcam). The detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation

(14) Checkpoint Kinase 2 Inhibition Can Reverse Tamoxifen Resistance in ER-Positive Breast CancerInternational Journal of Molecular SciencesOctober 14, 2022Ho Tsoi, Wai-Chung Tsang, Ellen P. S. Man, Man-Hong Leung, Chan-Ping You, Sum-Yin Chan, Wing-Lok Chan, Ui-Soon KhooUSA). ROS level was determined by CM-H2DCFDA (C6827; ThermoFisher, Waltham, MA, USA). TUNEL assay kit (ab66108; Abcam, Cambridge, UK) was employed to determine DNA damage. Absorbance and fluorescent signals were

(15) Dual specificity phosphatase 1 attenuates inflammation-induced cardiomyopathy by improving mitophagy and mitochondrial metabolismMolecular MetabolismOctober 1, 2022Ying Tan et al.(#ab232589, Abcam) was used at a 1:500 dilution. TUNEL staining was performed with a TUNEL Assay Kit (FITC; #ab66108, Abcam) as we previously described [14]. 4.12 Mitochondrial membrane potential, mitochondrial ROS and

(16) Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinomaWorld Journal of Surgical OncologyAugust 25, 2022Enqi Zheng, Zhun Cai, Wangyong Li, Chuandou Ni, Qian Fangusing an Olympus BX41 fluorescence microscope. Image quantification is analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously. MTT MTT assays are performed to assess cell proliferation

(17) Hyperglycemia aggravates ischemic brain damage via ERK1/2 activated cell autophagy and mitochondrial fissionFront Endocrinol (Lausanne)August 5, 2022Ping Liu, Xiao Yang, Jianguo Niu, Changchun Heiexperiment. Professor Andy Li assisted this part. TUNEL assay According to the manufacture’s instructions (ab66108,Abcam), the brain cells were rinsed with 1 x PBS, incubated in ice with membrane penetrating solution,

(18) TIA1 Loss Exacerbates Fatty Liver Disease but Exerts a Dual Role in HepatocarcinogenesisCancers (Basel)March 27, 2022Dobrochna Dolicka, Szabolcs Zahoran, Marta Correia de Sousa, Monika Gjorgjieva, Christine Sempoux, Margot Fournier, Christine Maeder, Martine A. Collart, Michelangelo Foti, Cyril Sobolewskithe day of the analysis, the cells were washed and stained as described in the TUNEL FITC Assay Kit (ab66108, Abcam, Cambridge, UK). The cells were then analyzed on CytoFLEX (Beckman Coulter, Brea, CA, USA) and

(19) Profiling Temporal Changes of the Pineal Transcriptomes at Single Cell Level Upon Neonatal HIBDFrontiers in Cell and Developmental BiologyMarch 8, 2022Xin Ding et al.detected no fluorescent signals. TUNEL assay was performed according to the manual (TUNEL-FITC, Abcam, ab66108). DNase I treated or PBS treated sildes were used as a positive and negative control of TUNEL staining,

(20) The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinomaBioengineeredMarch 1, 2022Liu X, Ma H, Ma L, Li K, Kang Y.end labeling (TUNEL) staining Tissue fractions from each group were analyzed using a TUNEL assay (ab66108, Abcam, Cambridge, UK), as described previously [21]. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide

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TUNEL Assay Kit - FITC from Abcam

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TUNEL Assay Kit - FITC from Abcam

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