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Calpain-Glo Protease Assay
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Calpain-Glo Protease Assay
Calpain-Glo Protease Assay
Calpain-Glo Protease Assay from Promega
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Citations:
(9)
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Calpain-Glo Protease Assay
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Description
The Calpain-Glo Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (µ) and 2 (m) activities
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Calpain-Glo Protease Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8502
Detection Target
Calpain
Quantity
50ml
Method
Luminescence
Molecular Weight
~95kDa
Type
Enzyme
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (9)
(1)
Protective Effects of Estrogen via Nanoparticle Delivery to Attenuate Myelin Loss and Neuronal Death after Spinal Cord Injury
Neurochemical Research
November 1, 2021
Azizul Haque, Kelsey P. Drasites, April Cox, Mollie Capone, Ali I. Myatich, Ramsha Shams, Denise Matzelle, Dena P. Garner, Mikhail Bredikhin, Donald C. Shields,
et al.
in triplicate in 96-well flat-bottomed plates using
Calpain-Glo™ Protease Assay
Kit (
Promega
G8501
,
G8502
) following manufacturer’s instructions. Plates were incubated at 37 °C for 5 min as indicated.
Luminescence
(2)
PAR-4/Ca(2+)-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetes
Cardiovascular diabetology
April 3, 2021
Giannella A, Ceolotto G, Radu CM, Cattelan A, Iori E, Benetti A, Fabris F, Simioni P, Avogaro A, Vigili de Kreutzenberg S.
Italy). Calpain activity
assay
Calpain activity was determined by
Calpain-Glo™ Protease Assay
(cat#
G8502
,
Promega
, Madison, USA) according to the manufacturer’s instructions. Briefly, washed
platelet
(400
(3)
Cisplatin induced neurotoxicity is mediated by Sarm1 and calpain activation
Scientific Reports
December 14, 2020
Aysel Cetinkaya-Fisgin, Xinghua Luan, Nicole Reed, Ye Eun Jeong, Byoung Chol Oh, Ahmet Hoke
proportional with
luminescence
was measured with a luminometer plate reader. Since Calpain-Glo (
Promega
,
G8501
) is designed for purified enzyme activity, we modified the original protocol to detect calpain activity
(4)
DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B
PLOS ONE
November 29, 2018
Mina Yousefelahiyeh, Jingyi Xu, Estibaliz Alvarado, Yang Yu, David Salven, Robert M. Nissen
(3483-12-3, Thermo Fisher Scientific), CaCl2 (10035-04-8, Fisher Scientific),
Calpain-Glo Protease Assay
(
G8501
,
Promega
). Antibodies used were anti-WDR68 (
HPA022948
,
Sigma-Aldrich
), anti-
DYRK1A
(
8765S
,
Cell Signaling
), anti-
DYRK1B
(5)
Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism
Scientific reports
June 22, 2018
Han H, Chou CC, Li R, Liu J, Zhang L, Zhu W, Hu J, Yang B, Tian J.
calpain 1 and 2 protease activity was evaluated using a Calpain-GloTM protease
assay
kit (
Promega
,
G8501
). Briefly, MDA-MB-231, PC-3 and LNCaP cells were seeded into 24-well plates and then treated with a
(6)
Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage
Scientific reports
February 20, 2018
Scott IC, Majithiya JB, Sanden C, Thornton P, Sanders PN, Moore T, Guscott M, Corkill DJ, Erjefält JS, Cohen ES.
Calpain protease activity in BAL samples was quantified using Calpain-Glo
luminescent assay
(
Promega
G8501
) according to manufacturer’s instructions. Biochemical
IL-33
assays
Human
rFL
IL-33
lysate, mock lysate
(7)
Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease
EBioMedicine
September 1, 2017
White MM, Geraghty P, Hayes E, Cox S, Leitch W, Alfawaz B, Lavelle GM, McElvaney OJ, Flannery R, Keenan J,
et al
.
quantified in
neutrophil
cytosols
using
Calpain-Glo™ Protease Assay
(
Promega
, WI, USA, product code
G8501
) (Reeves et al., 2013). Reagents were prepared and the protocol performed as per the manufacturer's
(8)
A calcium- and calpain-dependent pathway determines the response to lenalidomide in myelodysplastic syndromes
Nature medicine
July 1, 2016
Fang J, Liu X, Bolanos L, Barker B, Rigolino C, Cortelezzi A, Oliva EN, Cuzzola M, Grimes HL, Fontanillo C,
et al
.
(Bio-Rad). Calpain activity Calpain activity was measured according to the manufacturer’s instructions (
G8501
;
Promega
). Briefly, cells were washed in cold calcium-free 1× PBS and lysed in 10 mM HEPES (pH 7.2),
(9)
The combination of lithium and l-Dopa/Carbidopa reduces MPTP-induced abnormal involuntary movements (AIMs) via calpain-1 inhibition in a mouse model: Relevance for Parkinson׳s disease therapy
Brain Research
October 5, 2015
Carol A. Lazzara, Rebeccah R. Riley, Anand Rane, Julie K. Andersen, Yong-Hwan Kim
calpain 1 and 2 activities and other steps were followed as recommended by the manufacturer (
Promega
G8501
, Madison, WI). Due to the limit of collecting high concentration of total protein from the midbrain
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Calpain-Glo Protease Assay from Promega
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