Amylase Assay Kit (Colorimetric) ab102523 from Abcam

Product Specs
ItemAmylase Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab102523
Quantity100 test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
Assay Time0h 40m
Sensitivity0.2 mU/well
ApplicationsCellular Assay
ReactivityMammals, Mammals, Other species
Detection TargetAmylase

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Galantamine ameliorates experimental pancreatitisMolecular MedicineOctober 31, 2023Dane A. Thompson, Tea Tsaava, Arvind Rishi, Sam J. George, Tyler D. Hepler, Daniel Hyde, Valentin A. Pavlov, Michael Brines, Sangeeta S. Chavan, Kevin J. Traceyreported as a total score. Serum amylase activity was measured via colorimetric enzymatic assay (Abcam, ab102523). Serum and pancreatic cytokine concentrations were measured by ELISA (Meso Scale Diagnostics, V-Plex). Statistical

(2) Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of ButyrateNutrientsOctober 18, 2023He Xia, Jing Guo, Jian Shen, Shiman Jiang, Shengyi Han, Lanjuan Li(ab269542, Abcam, Cambridge, UK). The serum amylase and lipase were detected by the Amylase Assay Kit (ab102523, Abcam, Cambridge, UK) and the Lipase Assay Kit (ab102524, Abcam, Cambridge, UK). Superoxide dismutase

(3) Sustainable Aquafeed Formulations Containing Insect Larval Meal and Grape Marc for the New Zealand Farmed AbaloneAquaculture NutritionOctober 11, 2023Natalia Bullon, Andrea C. Alfaro, Moganakumaar Manivannan, Seyedehsara Masoomi Dezfooli, Ali SeyfoddinAmylase was determined at 405 nm at 0 and 3 min due to the high activity of the samples (Catalogue AB102523; Abcam, Australia) in a kinetic mode. Lipase was determined at 412 nm at 0 and 30 min (Catalogue AB102525;

(4) Copper-dependent autophagic degradation of GPX4 drives ferroptosisAutophagyJuly 1, 2023Xue Q, Yan D, Chen X, Li X, Kang R, Klionsky DJ, Kroemer G, Chen X, Tang D, Liu J.Stain Kit,Thermo Fisher Scientific,"87,006" Iron Assay Kit,Abcam,ab83326 Amylase assay kit,Abcam,ab102523 HMGB1 ELISA kit,Sino-Test Corporation,"326,070,442" MPO ELISA kit,Thermo Fisher Scientific,EMMPO FerroOrange,DOJINDO,F347 Evo

(5) Optogenetic stimulation of the brainstem dorsal motor nucleus ameliorates acute pancreatitisFrontiers in ImmunologyApril 25, 2023Dane A. Thompson, Tea Tsaava, Arvind Rishi, Sandeep Nadella, Lopa Mishra, David A. Tuveson, Valentin A. Pavlov, Michael Brines, Kevin J. Tracey, Sangeeta S. Chavanexperimental groups. Assays Serum amylase activity was measure via colorimetric enzymatic assay (Abcam, ab102523). Serum and pancreatic cytokine levels were measured with enzyme-linked immunosorbent assay (ELISA)

(6) Cananga odorata Aromatherapy Reduces Anxiety in Unexperienced Patients Hospitalized for Interventional Neuroradiology Procedures: A Randomized Control TrialJournal of Evidence-Based Integrative MedicineJanuary 20, 2023Jetsada Sriboonlert, Waranon Munkong, Sunantha Rintawut, Soodjai Paladkhua, Ratchalita Suwongsa, Woranan Kirisattayakulthe unit of ng/ml. The salivary alpha-amylase activity was determined using amylase assay kit Abcam ab102523 (Cambridge, United Kingdom) by colorimetric method following the protocol book of the product. Salivary

(7) Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosisAutophagyJanuary 1, 2023Chen X, Song X, Li J, Zhang R, Yu C, Zhou Z, Liu J, Liao S, Klionsky DJ, Kroemer G, et al.Assays,Critical Commercial Assays 4%-12% Criterion XT Bis-Tris gels,Bio-Rad,"3,450,124" Amylase Assay Kit,Abcam,ab102523 BCA Assay Kit,Thermo Fisher Scientific,23225 CCK8,Bimake,B34304 Cell fractionation kit,Cell Signaling Technology

(8) DCN released from ferroptotic cells ignites AGER-dependent immune responsesAutophagySeptember 1, 2022Liu J, Zhu S, Zeng L, Li J, Klionsky DJ, Kroemer G, Jiang J, Tang D, Kang R.ab105136), LDH (Abcam, ab102526), trypsin (Abcam, ab102531), CTSB (Abcam, ab119585), and AMY (Abcam, ab102523) in the indicated samples. RNAi All shRNA constructs in a lentiviral format as described in Table

(9) Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion InjuryFrontiers in PhysiologyJune 23, 2022Asmaa Mohammed ShamsEldeen, Sarah A. Abd El-Aal, Basma Emad Aboulhoda, Hend AbdAllah, Sara Mahmoud Gamal, Fatma E. Hassan, Marwa Nagi Mehesen, Laila Ahmed Rashed, Abeer Mostafa, Nermeen Bakr SadekAmylase, LDH, and MPO Serum amylase was measured using Colorimetric assay kits obtained from Abcam (ab102523) following the manufacturer’s instructions. Lactate dehydrogenase Enzyme (LDH) Activity Assay: Tissue

(10) Negative Immune Checkpoint Protein, VISTA, Regulates the CD4+ Treg Population During Sepsis Progression to Promote Acute Sepsis Recovery and SurvivalFrontiers in ImmunologyMarch 24, 2022Chyna C. Gray, Bethany Biron-Girard, Michelle E. Wakeley, Chun-Shiang Chung, Yaping Chen, Yael Quiles-Ramirez, Jessica D. Tolbert, Alfred AyalaKit (Sigma-Aldrich: cat# MAK055), Amylase Assay Kit (Colorimetric) (Abcam, Cambridge, MA, USA: cat# ab102523), and Bilirubin Assay Kit (Direct Colorimetric) (Abcam: cat# ab235627). 2.6 Multiplex Cytokine Analysis Plasma

(11) Reduction in O-GlcNAcylation Mitigates the Severity of Inflammatory Response in Cerulein-Induced Acute Pancreatitis in a Mouse ModelBiology (Basel)February 22, 2022Mackenzie Moore, Nandini Avula, Alicia Wong, Megan Beetch, Seokwon Jo, Emilyn U. AlejandroCytokine Reference Lab at the University of Minnesota (MAGPIX, Luminex, Austin, TX, USA). Amylase (Abcam, ab102523), lipase (Sigma-Aldrich, MAK046), and LDH (Sigma-Aldrich, MAK066) activity assays were performed according

(12) Herbivorous Juvenile Grass Carp (Ctenopharyngodon idella) Fed with Genetically Modified MON 810 and DAS-59122 Maize Varieties Containing Cry Toxins: Intestinal Histological, Developmental, and Immunological InvestigationsToxins (Basel)February 19, 2022Gergő Gyurcsó, Béla Darvas, Ferenc Baska, László Simon, Eszter Takács, Szandra Klátyik, András Székácsα-Amylase The level of α-amylase activity was detected by colorimetry using the Amylase Activity Assay kit (No: ab102523; Abcam, Cambridge, UK) and following the manufacturer’s protocol with a sensitivity of 0.2 mU/well. 4.8.2.

(13) Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costataMetabolitesFebruary 9, 2022Martin Moos, Jaroslava Korbelová, Tomáš Štětina, Stanislav Opekar, Petr Šimek, Robert Grgac, Vladimír Koštáltaken in 3 replicates) were homogenized in 1 mL of Assay Buffer supplied in the Amylase Assay Kit (ab102523, Abcam, Cambridge, UK) and 50 µL aliquots of supernatant were used as sources of enzymatic activity.

(14) Trypsin-Mediated Sensitization to Ferroptosis Increases the Severity of Pancreatitis in MiceCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Liu K, Liu J, Zou B, Li C, Zeh HJ, Kang R, Kroemer G, Huang J, Tang D.Western blot. Enzyme-Linked Immunosorbent Assay Analysis The concentrations or activity of amylase (ab102523; Abcam), MPO (EMMPO; Thermo Fisher Scientific), trypsin (ab102531; Abcam), 8-hydroxy-2-deoxyguanosine

(15) RORγt inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL‐17‐producing Th17 and γδ‐T cells in mice with ceruletide‐induced pancreatitisBasic & Clinical Pharmacology & ToxicologyNovember 1, 2021Jianfa Wang, Yayun Xu, Hui Jing, Qimeng Chang, Xubo Wu, Ziping ZhangIL‐23 (EK123, A12301234, EK282, A28210345) in all mouse and human blood samples and serum amylase (ab102523; Abcam) in mice were measured with the corresponding ELISA kits. 2.10 Real‐time PCR RNA in PBMCs was

(16) Chlorogenic acid reduces inflammation in murine model of acute pancreatitisPharmacological ReportsOctober 1, 2021Aleksandra Tarasiuk, Kamila Bulak, Marcin Talar, Jakub Fichnaenzyme. Determination of amylase activity in the pancreas Amylase Assay Kit (Colorimetric) (Abcam, ab102523) was used to detect activity of α-amylase through a two-step reaction, according to manufacturer’s protocol. Histology Pancreas

(17) Ghee Butter from Bovine Colostrum Reduces Inflammation in the Mouse Model of Acute Pancreatitis with Potential Involvement of Free Fatty Acid ReceptorsNutrientsSeptember 19, 2021Tarasiuk A, Talar M, Bulak K, Fichna J.triplicate. 2.7. Determination of Amylase Activity in Blood Serum The Amylase Assay Kit (Colorimetric) (Abcam, ab102523) was used to quantify α-amylase activity through a two-step reaction. In the protocol, the substrate

(18) Diabetic Bone Marrow Cell Injection Accelerated Acute Pancreatitis ProgressionJournal of Immunology ResearchAugust 21, 2021Xiao-Min Luo, Cen Yan, Yue-Jie Zhang, Ling-Jia Meng, Guo-Tao Lu, Ji-Ming Yin, Ying-Mei Fengmeasured by commercial kits and quantified by a Tecan Safire microplate reader (mouse amylase assay kit: ab102523, Abcam, Cambridge, MA; lipase kits: Nanjing Jiancheng Corp., Nanjing, China). 2.5. Cytokine Measurement Proteins

(19) Do proinflammatory cytokines play a role in clozapine-associated glycometabolism disorders?Psychopharmacology (Berl)July 1, 2021Tongtong Zhao et al.for ELISA. The amylase levels in the serum were measured using the amylase assay colorimetric kit (ab102523) from Abcam (Cambridge, UK), and the level of amylase was recorded in mU/ml (nmol/min/ml). The serum

(20) Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathwayNature communicationsDecember 11, 2020Dai E, Han L, Liu J, Xie Y, Zeh HJ, Kang R, Bai L, Tang D.Mycoplasma-negative and were characterized by short tandem repeat profiling. ELISA analysis The amylase (#ab102523, Abcam), myeloperoxidase level (#EMMPO, Thermo Fisher Scientific), trypsin (#ab102531, Abcam), 4-HNE

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Amylase Assay Kit (Colorimetric) from Abcam

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Amylase Assay Kit (Colorimetric) from Abcam

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