Fig 1: QRHX inhibits PI3K/Akt signal pathway and down-regulates the level of inflammatory cytokines. (A) Inflammatory factor levels of MCP-1, IL-17A, TNF-a and IL-1ß. (B) Protein expression levels of p-PI3K, PI3K, p-Akt and Akt. (C) Quantitative analysis of p-PI3K, PI3K, p-Akt and Akt using ImageJ software. Data were expressed as mean ± SEM. **p < 0.01 vs. Normal group, #p < 0.05 and ##p < 0.01 vs. Model group.
Fig 2: Markers of hepatic function, inflammation and fibrosis. a After 16 weeks ALAT-levels were significantly higher in NASH-fed rats compared to Control-, HFD- and HFr-fed rats. b Plasma levels of ASAT were higher in the NASH-group compared to both Control and HFr-groups. c Rats on NASH, HFD and HFr-diet had increased hepatic levels of MCP-1 compared to Control. d Hepatic TNF-a levels were significantly increased in the HFD fed rats compared to Control. e Haptoglobin was significantly increased in plasma of NASH-fed rats compared to rats fed Control, HFD and HFr. f Plasma TIMP-1 was significantly increased in the NASH-fed rats, compared to all other groups. Statistical significance: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Results are shown as mean ± SEM
Fig 3: Effect of high salt (HS), l-buthionine-sulfoximine (BSO) and resveratrol (R) on renal inflammatory markers. Vehicle (V) rats were kept on tap water. The levels of (A) interleukin 1ß (IL-1ß), (B) interleukin 6 (IL-6), (C) tissue necrosis factor a (TNF-a), (D) monocyte chemoattractant protein 1 (MCP-1), (E) granulocyte macrophage colony-stimulating factor (GM-CSF), and (F) interleukin 10 (IL-10) were measured in renal proximal tubules after 6-wk treatment by commercially available kits. Experiments were performed in quadruplicate (n=6–8 rats). *P<0.05 vs V by 1-way ANOVA followed by a post hoc Newman–Keuls test.
Supplier Page from Abcam for Rat MCP1 ELISA Kit (CCL2)