Fig 1: CX3CL1 derived from BMSCs statistically augmented Aß1-42-induced SH-SY5Y viability but repressed apoptosis. (a) CCK-8 assay assessed SH-SY5Y viability. (b and c) Flow cytometry evaluated SH-SY5Y apoptosis. (d and e) Western blotting determined cleaved caspase-3 expression relative to ß-actin. *p < 0.05 versus control group; #p < 0.05 versus Aß group; &p < 0.05 versus Aß+MSC group.
Fig 2: CX3CL1 derived from BMSCs alleviated the pathology-induced Aß1-42 in SH-SY5Y. (a–d) Western blotting examined Aß, tau, and p-Tau protein expressions relative to ß-actin. (e and f) The relative integrated density of Iba1 was evaluated by IF. (g) The axons of SH-SY5Y cells were observed using inverted microscope. (h–k) Western blotting examined SNAP25, Synapsin1, and PSD95 protein levels relative to ß-actin. *p < 0.05 versus control group; #p < 0.05 versus Aß group; &p < 0.05 versus Aß+MSC group.
Fig 3: CX3CL1 derived from BMSCs significantly inverted Aß1-42-decreased CX3CL1 in SH-SY5Y. (a) Soluble CX3CL1 expression in BMSCs was detected by ELISA. (b and c) Western blotting determined CX3CL1 expression relative to ß-actin in BMSCs. (d) ELISA measured soluble CX3CL1 levels in SH-SY5Y under different interventions. (e and f) Western blotting examined CX3CL1 expression relative to ß-actin in SH-SY5Y under different interventions. ns: nonsignificance; *p < 0.05 versus control group; #p < 0.05 versus Aß group; &p < 0.05 versus Aß+MSC group.
Fig 4: The average concentration of the CX3CL1 in serum (a) and synovial fluid (b) obtained from patients. The data has been presented as a mean value ± standard deviation (SD). *p < 0.05. OA: osteoarthritis; HA: hemophilic arthropathy.
Fig 5: The role played by CX3CL1 derived from BMSCs in Aß1-42-induced SH-SY5Y was related to TXNIP/NLRP3 axis. (a–d) MDA, SOD, CAT, and GSH-Px concentrations measured by commercial kits. (e–h) Western blotting tested TXNIP, NLRP3, and Trx protein levels relative to ß-actin. *p < 0.05 versus control group; #p < 0.05 versus Aß group; &p < 0.05 versus Aß+MSC group.
Supplier Page from Abcam for Human Fractalkine ELISA Kit (CX3CL1)