Fig 1: SENP3 promotes the expression of proinflammatory factors in microglia after cerebral I/R injury(A) Primary cultured microglia were transduced with adenovirus expressing empty vector (Ad-vector), SENP3 coding sequence (Ad-SENP3), scramble (Ad-sh.NC) or SENP3 shRNA sequence (Ad-sh.SENP3), and then treated with OGD/R. The mRNA level of Il-ß, Il-6, Tnf-a, Cxcl1 and Ccl2 in microglial cells was quantified by RT-qPCR assay.(B) The secretion level for IL-ß, IL-6, TNF-a, CXCL1 and CCL2 in primary microglia supernatants was detected by ELISA analysis.(C and D) The protein expression of iNOS and CD16/32 was detected by immunoblots assay (C) and the analysis of relative band intensity was depicted (D). n = 3 per group.(E) The intensity of iNOS (Green) and Iba-1 (Red) in primary cultured microglia was determined by immunofluorescence assays. Scale bars: 20 µm.(F) The fluorescence intensity of iNOS and Iba-1 was analyzed via ImageJ software. Data are presented as means ± SEM and analyzed by one-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Supplier Page from Abcam for Mouse CXCL1 ELISA Kit (GRO alpha)