Fig 1: Phospho-ERK (pERK) immunostaining and fluorescence in situ hybridization (FISH) for BRAF.Validation of the pERK antibody using NIH/3T3 cells that are either untreated (A) or PMA-treated (B). C. pERK IHC of BT06 PXA with a BRAF V600E mutation (IHC semi-quantitative score 1). D. BT23 PXA without BRAF V600E mutation (IHC semi-quantitative score of 2). E. BT24 PXA with a BRAF V600E mutation (IHC semi-quantitative score 4). F. BT25 PXA without BRAF V600E mutation (IHC semi-quantitative score of 4). G. BRAF FISH with probes for CEP7 (centromeric control probe - aqua), 5'BRAF (green) and 3'BRAF (red). PXA BT29 demonstrating no duplication of the BRAF locus and chromosome 7 disomy. H. PXA BT41, which is the recurrence of BT29, did not have duplication of the BRAF locus but demonstrated chromosome 7 polysomy.
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