Fig 1: Src inhibitor-1 blocks encystment process and GTP-RhoA activity. a Percentage of cyst formation inhibition by Src inhibitor-1 treatment (30 µM) during 96 h. Calcofluor m2r staining of cysts produced in each condition. b E. invadens trophozoites were induced to encyst in the presence of Src inhibitor-1 or vehicle (DMSO); after 12 h of encystment, the level of RhoA-GTP was determined with a G-LISA RhoA activation assay kit (Kit no. BK121, Cytoskeleton, Inc., Denver, CO, USA), according to the manufacturer’s instructions
Fig 2: Increase in RhoA-GTP levels at early times of encystment. RhoA-GTP increased at early times but decreased later on during encystment. RhoA-GTP levels were determined by G-LISA RhoA activation assay kit (Kit no. BK121, Cytoskeleton, Inc., Denver, CO). Results are the average of three independent experiments done in duplicate (*P < 0.05).
Supplier Page from Cytoskeleton, Inc. for RhoA G-LISA Activation Assay (luminescence)