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Products
Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
RhoA ELISA Kit
RhoA ELISA Kit from Cytoskeleton, Inc.
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Citations:
(14)
Supplier Page
Supplier Page from
Cytoskeleton, Inc. for
RhoA ELISA Kit
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
RhoA ELISA Kit
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
BK150
Detection Target
RhoA
Quantity
KIT 96 assays
Method
Rho G-LISA Assay Kits
Applications
ELISA
Brand/Trademark
Cytoskeleton, Inc
Add to Compare List
Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (14)
(1)
Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome
Journal of translational medicine
June 18, 2023
Zhai R, Lenga Ma Bonda W, Leclaire C, Saint-Béat C, Theilliere C, Belville C, Coupet R, Blondonnet R, Bouvier D, Blanchon L,
et al
.
results were normalized to the total RhoA level as measured using the Total RhoA
ELISA
Biochem Kit (
BK150
,
Cytoskeleton
, Denver, USA). Active RhoA was determined in duplicate with the same colorimetric RhoA
(2)
Dual mechanism underlying failure of neural tube closure in the Zic2 mutant mouse
Disease Models & Mechanisms
March 1, 2023
Sarah Escuin, Saba Rose Rose Raza-Knight, Dawn Savery, Carles Gaston-Massuet, Gabriel L. Galea, Nicholas D. E. Greene, Andrew J. Copp
instructions (Escuin et al., 2015). Total RhoA was determined by
enzyme-linked immunosorbent assay
(
ELISA
;
BK150
,
Cytoskeleton Inc
.).
Assays
were performed in duplicate on three biological replicates for each genotype
(3)
AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence
Nature
September 1, 2021
Lee S, Karki R, Wang Y, Nguyen LN, Kalathur RC, Kanneganti TD.
Cytoskeleton
) and normalized to total RhoA levels, which were measured using the Total
RhoA ELISA kit
(
BK150
,
Cytoskeleton
) according to the manufacturer’s instructions. For
pull-down
assays
, a GST-tagged Rho
(4)
DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular Calcification
Arteriosclerosis, Thrombosis, and Vascular Biology
July 1, 2020
David Ngai, Marsel Lino, Katheryn E. Rothenberg, Craig A. Simmons, Rodrigo Fernandez-Gonzalez, Michelle P. Bendeck
Cytoskeleton
) and normalized to total RhoA measured by RhoA E-LISA (
enzyme-linked immunosorbent assay
;
BK150
;
Cytoskeleton
) according to the manufacturer’s instructions.
Immunocytochemistry
To assess stiffness-dependent
(5)
Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway
Nature materials
June 1, 2020
Chronopoulos A, Thorpe SD, Cortes E, Lachowski D, Rice AJ, Mykuliak VV, Róg T, Lee DA, Hytönen VP, Del Río Hernández AE.
RhoA-GTP were quantified using total RhoA
ELISA
and active RhoA G-LISA
assay
kits (
Cytoskeleton, Inc
.
BK150
and
BK124
) according to the manufacturer’s instructions. Immediately
after treatment
, cells were rinsed
(6)
Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons
PLoS biology
September 1, 2019
Haq N, Schmidt-Hieber C, Sialana FJ, Ciani L, Heller JP, Stewart M, Bentley L, Wells S, Rodenburg RJ, Nolan PM,
et al
.
(
Cytoskeleton
,
BK128
). The activity of RhoA was normalised to the total level of RhoA (
Cytoskeleton
,
BK150
).
Immunofluorescence
and image acquisition Primary hippocampal cultures were prepared from E16.5 C57BL/6Ntac
(7)
Nano-topography: Quicksand for cell cycle progression?
Nanomedicine: Nanotechnology, Biology and Medicine
November 1, 2018
Marianna Giannini, Chiara Primerano, Liron Berger, Martina Giannaccini, Zhigang Wang, Elena Landi, Alfred Cuschieri, Luciana Dente, Giovanni Signore, Vittoria Raffa
extracted and chilled in N2. After normalization of protein concentration, quantification of total RhoA (
BK150
,
Cytoskeleton Inc
.) and active RhoA (
BK124
,
Cytoskeleton Inc
.) was performed in the same lysates.
Scanning electron microscopy
(8)
Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet
Frontiers in pharmacology
January 1, 2018
Peng JH, Leng J, Tian HJ, Yang T, Fang Y, Feng Q, Zhao Y, Hu YY.
G-LISA RhoA activation assay biochem kit (
BK124
,
Cytoskeleton, Inc
., CO, US), and total
RhoA ELISA kit
(
BK150
,
Cytoskeleton, Inc
., CO, US), respectively. The colon tissue sample was prepared according to the instruction
(9)
Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: their emerging role in tumor heterogeneity
Scientific Reports
December 1, 2017
Odessa Schillaci, Simona Fontana, Francesca Monteleone, Simona Taverna, Maria Antonietta Di Bella, Dolores Di Vizio, Riccardo Alessandro
was quantified by measuring the amounts of RhoA-GTP via the G-LISA RhoA Activation Biochem
assay
kit (
BK150
Cytoskeleton, Inc
, Denver, CO), an
enzyme-linked immunosorbent assay
(
ELISA
)-based. The total cellular
(10)
Rho-associated kinase is a therapeutic target in neuroblastoma
Proceedings of the National Academy of Sciences
August 8, 2017
Cecilia Dyberg et al.
Activity
Assays
. Total RhoA, active RhoA, and active Rac1 were investigated using
ELISA
and G-LISA kits (
BK150
,
BK124
, and
BK128
;
Cytoskeleton
). The RhoA G-LISA kit uses a plate coated with Rho-GTP– binding protein,
(11)
Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
Nature immunology
August 1, 2016
Park YH, Wood G, Kastner DL, Chae JJ.
(
BK121
,
cytoskeleton
) and normalized to total
RhoA
levels, which were measured by Total
RhoA ELISA kit
(
BK150
,
Cytoskeleton
).
RhoA
-GTP levels of BMDMs treated with C3 toxin (0.5 μg/ml) and colchicine (102 – 104
(12)
Rho kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure
Journal of Cell Science
July 15, 2015
Sarah Escuin, Bertrand Vernay, Dawn Savery, Christine B. Gurniak, Walter Witke, Nicholas D. E. Greene, Andrew J. Copp
spectrophotometer reader (BioTek synergy HT). Total RhoA was determined by an ELISA assay (catalogue no.
BK150
,
Cytoskeleton Inc
.).
Assays
were performed with duplicate
measures
.
Immunohistochemistry
Gelatin was
(13)
Tetraspanin CD9 Regulates Cell Contraction and Actin Arrangement via RhoA in Human Vascular Smooth Muscle Cells
PLoS ONE
September 3, 2014
Michael J. Herr, Scott E. Mabry, Lisa K. Jennings
GTP-bound RhoA Active and GTP-bound RhoA were measured from cell lysates using a total RhoA
ELISA
(
BK150
) or RhoA activation G-LISA kit (
BK124
) purchased from
Cytoskeleton, Inc
. (Denver, CO) per the manufacturer's
(14)
The impact of flow-induced forces on the morphogenesis of the outflow tract
Frontiers in Physiology
June 17, 2014
Stefanie V. Biechler, Lorain Junor, Ashlie N. Evans, John F. Eberth, Robert L. Price, Jay D. Potts, Michael J. Yost, Richard L. Goodwin
as described previously (Tan et al., 2012) using colorimetric
ELISA
assays
(Total
RhoA ELISA Kit
, #
BK150
,
Cytoskeleton Inc
.; RhoA Activation Assay Biochem Kit, #
BK124
,
Cytoskeleton Inc
.). The percentage of
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RhoA ELISA Kit from Cytoskeleton, Inc.
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