CytoTox-ONE Homogeneous Membrane Integrity Assay G7890 from Promega

Product Specs
ItemCytoTox-ONE Homogeneous Membrane Integrity Assay
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberG7890
Detection TargetCell viability
Sample TypeCells
MethodFluorometric (resorufin)
Quantity200-800 assays
Assay Time10 minutes

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) The colonic pathogen Entamoeba histolytica activates caspase-4/1 that cleaves the pore-forming protein gasdermin D to regulate IL-1β secretionPLoS pathogensMarch 1, 2022Wang S, Moreau F, Chadee K.macrophage culture was measured with the Promega CytoTox-ONE homogeneous membrane integrity assay (G7890, Fisher Scientific) following the instruction from the manufacturer. Relative LDH release was calculated

(2) Tau interactome maps synaptic and mitochondrial processes associated with neurodegenerationCellFebruary 17, 2022Tracy TE, Madero-Pérez J, Swaney DL, Chang TS, Moritz M, Konrad C, Ward ME, Stevenson E, Hüttenhain R, Kauwe G, et al.Human Stem Cell Nucleofector Kit 1,Lonza,Cat#VPH-5012 CytoTox-ONE Homogeneous Membrane Integrity Assay,Promega,Cat#G7890 Image-iT TMRM Reagent,Invitrogen,Cat#I34361 Hoechst 33342,Invitrogen,Cat#H3570 Calcein-AM,Invitrogen,Cat#C3099 ,, Deposited

(3) Deep Learning Image Analysis of High-Throughput Toxicology Assay ImagesSLAS DiscoveryJanuary 21, 2022Arpit Tandon, Brian Howard, Sreenivasa Ramaiahgari, Adyasha Maharana, Stephen Ferguson, Ruchir Shah, B. Alex Merrickrelease was measured using CytoTox-ONE reagent following the manufacturer’s protocol (Promega, Catalog: G7890). LDH graphs are provided in the supplementary file S2. Cell viability assay data as shown in Figure

(4) Identification of an ASC oligomerization inhibitor for the treatment of inflammatory diseasesCell death & diseaseDecember 13, 2021Soriano-Teruel PM, García-Laínez G, Marco-Salvador M, Pardo J, Arias M, DeFord C, Merfort I, Vicent MJ, Pelegrín P, Sancho M, et al.the release of LDH according to a commercial kit (CytoTox-ONE™ Homogeneous Membrane Integrity Assay G7890; Promega). The release of LDH was calculated using the formula: the release of LDH (%) = 100 × (Abs490

(5) HIF‑1α mediates the protective effect of plasma extracellular particles induced by remote ischaemic preconditioning on oxidative stress injury in human umbilical vein endothelial cellsExperimental and Therapeutic MedicineNovember 15, 2021Minghua Wen, Feng Hu, Zhijian Gong, Chahua Huang, Xiaoshu Chengof cell injury, was quantified using a CytoTox-ONE™ Homogeneous Membrane Integrity Assay (cat. no. G7890; Promega Corporation) according to the manufacturer's protocol. Cell viability was determined by a Cell

(6) Acetate Does Not Affect Palmitate Oxidation and AMPK Phosphorylation in Human Primary Skeletal Muscle CellsFront Endocrinol (Lausanne)June 17, 2021Manuel A. González Hernández, Ellen E. Blaak, Nicole T. H. Hoebers, Yvonne P. G. Essers, Emanuel E. Canfora, Johan W. E. Jockendehydrogenase (LDH) assay was used for estimating the number of nonviable cells, as marker of cytotoxicity (#G7890, Promega). Differentiated cells were cultured without (0 mmol/L), or with physiological (0.1 mmol/L)

(7) Fast Stereolithography Printing of Large‐Scale Biocompatible Hydrogel ModelsAdvanced Healthcare MaterialsMay 1, 2021Nanditha Anandakrishnan et al.assay (Lactate dehydrogenase assay, CytoTox-ONE Homogeneous Membrane Integrity Assay kit from Promega, G7890) was used to determine the level of cytotoxicity caused by membrane compromisation, three different

(8) Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin HydrogelsBiomedicinesMarch 5, 2021Distler T, Lauria I, Detsch R, Sauter CM, Bendt F, Kapr J, Rütten S, Boccaccini AR, Fritsche E.cells and is indicative for cytotoxicity, using the CytoTox-ONE Homogeneous Membrane Integrity assay (#G7890, both Promega, Madison, WI, USA). All assays were performed in accordance with the manufacturer’s instructions.

(9) Mesoporous Bioactive Glasses Cytocompatibility Assessment: A Review of In Vitro StudiesBiomimetics (Basel)January 23, 2021Margaux Salètes, Marta Vartin, Caroline Mocquot, Charlène Chevalier, Brigitte Grosgogeat, Pierre Colon, Nina Attikmorphology: SEM & CLSMCell viability: Alamar Blue assay and membrane integrity: LDH: CytoTox-ONE ™ (Promega, G7890)Cell differentiation: ALP activity assay","MBG stimulated cell proliferation, colonization, and differentiation.Cell

(10) Synthesis and use of an amphiphilic dendrimer for siRNA delivery into primary immune cellsNature protocolsJanuary 1, 2021Chen J, Ellert-Miklaszewska A, Garofalo S, Dey AK, Tang J, Jiang Y, Clément F, Marche PN, Liu X, Kaminska B, et al.(Biowest, Product code: L0950) CytoTox-ONE™ Homogeneous Membrane Integrity Assay (Promega, cat no. G7890) MycoAlert Mycoplasma detection kit (Lonza, cat. no. LT07–318) Plasmocin (Invivogen, cat code. ant-mpt-1)

(11) The Lysosomal Membrane Protein Lamp2 Alleviates Lysosomal Cell Death by Promoting Autophagic Flux in Ischemic CardiomyocytesFrontiers in cell and developmental biologyJanuary 1, 2020Cui L, Zhao LP, Ye JY, Yang L, Huang Y, Jiang XP, Zhang Q, Jia JZ, Zhang DX, Huang Y.Beyotime), and cytotoxicity was detected with a CytoTox-ONETM C homogeneous membrane integrity assay kit (G7890, Promega, United States), which is a fluorometric method used to measure the amount of lactate dehydrogenase

(12) CD38 Causes Autophagic Flux Inhibition and Cardiac Dysfunction Through a Transcriptional Inhibition Pathway Under Hypoxia/Ischemia ConditionsFrontiers in cell and developmental biologyJanuary 1, 2020Zhang X, Li L, Zhang Q, Wei Q, Lin J, Jia J, Zhang J, Yan T, Lv Y, Jiang X, et al.using a CytoTox-ONETM Homogeneous Membrane Integrity Assay Kit (Promega, Madison, WI, United States, G7890). CM viability was measured by Cell Counting Kit-8 (CCK-8) assay. Sirt1 and Sirtuins Activity Measurements Sirt1

(13) Dissecting the Anticancer Mechanism of Trifluoperazine on Pancreatic Ductal AdenocarcinomaCancersNovember 26, 2019Huang C, Lan W, Fraunhoffer N, Meilerman A, Iovanna J, Santofimia-Castaño P.caspase-3/7 activity were measured using CellTiter-Glo (#G7571, Promega, Madrid, Spain), CytoTox-ONE (#G7890, Promega), and Caspase-Glo 3/7 assay (#G8091, Promega). The normalization of the data was done by the

(14) Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal AntagonistJournal of immunology (Baltimore, Md. : 1950)November 15, 2019Chen Y, Wang H, Shen J, Deng R, Yao X, Guo Q, Lu A, Sun B, Zhang Y, Meng G.(catalog no. 88-7324) were from Thermo Fisher Scientific. The lactate dehydrogenase (LDH) kit (catalog no. G7890) was purchased from Promega. 2713 2714 INFLAMMASOME EFFECTOR GALECTIN-3 CAUSES INSULIN RESISTANCE were

(15) Acute Cytotoxic Effects on Morphology and Mechanical Behavior in MCF-7 Induced by TiO2NPs ExposureInternational Journal of Molecular SciencesJuly 23, 2019Mariafrancesca Cascione, Valeria De Matteis, Giacomo Mandriota, Stefano Leporatti, Rosaria Rinaldidehydrogenase (LDH) leakage assay, using the CytoTox-ONE Homogeneous Membrane Integrity Assay reagent (G7890, Promega, Madison, WI, USA), were performed to conduct viability testing and to evaluate the membrane

(16) AgCuB nanoparticle eradicates intracellular S. aureus infection in bone cells: in vitroEmergent MaterialsJune 1, 2019Shahnaz Qadri, Tahir Abdulrehman, Jamil Azzi, Said Mansour, Yousef Haikpositive and negative controls. 2.13 LDH assay CytoTox-ONE assay kit was purchased from Promega Cat # G7890 to measure the lactate dehydrogenase (LDH). The protocol was followed as directed by the manufacturer.

(17) The Power of Resolution: Contextualized Understanding of Biological Responses to Liver Injury Chemicals Using High-throughput Transcriptomics and Benchmark Concentration ModelingToxicological SciencesJune 1, 2019Sreenivasa C Ramaiahgari et al.release was measured using CytoTox-ONE reagent following the manufacturer’s protocol (Promega, Catalog: G7890). TempO-Seq analysis. At the conclusion of the 96 h compound exposure period, exposure media were removed

(18) Epoxyscillirosidine Induced Cytotoxicity and Ultrastructural Changes in a Rat Embryonic Cardiomyocyte (H9c2) Cell LineToxins (Basel)May 21, 2019Hamza Ibrahim Isa, Gezina Catharina Helena Ferreira, Jan Ernst Crafford, Christoffel Jacobus BothaBE-17-605E) were acquired from Lonza (Verviers, Belgium). The LDH assay kit (CytoTox-ONETM , cat. no: G7890) was purchased from Promega Corp., USA. Dulbecco’s Modified Eagle’s Medium (DMEM, cat. no: D6546), phosphate

(19) ADAM17-dependent signaling is required for oncogenic human papillomavirus entry platform assemblyeLifeMay 20, 2019Mikuličić S, Finke J, Boukhallouk F, Wüstenhagen E, Sons D, Homsi Y, Reiss K, Lang T, Florin L.#: 589904, "Commercial assay, kit",CytoTox-ONE Homogeneous Membrane Integrity Assay,Promega,Cat. #: G7890, "Commercial assay, kit",DuolinkIn Situ Orange Starter Kit Mouse/Rabbit,Sigma-Aldrich,Cat. #: DUO92102, "Commercial

(20) Ligand-based design identifies a potent NUPR1 inhibitor exerting anticancer activity via necroptosisJournal of Clinical InvestigationMay 13, 2019Patricia Santofimia-Castaño et al.experiment, LDH release, ATP production, and caspase 3/7 activity were monitored using CytoTox-ONE (Promega G7890), CellTiter-Glo (Promega G7571) and Caspase-Glo 3/7 (Promega G8091) assays, respectively. Data were

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CytoTox-ONE Homogeneous Membrane Integrity Assay

CytoTox-ONE Homogeneous Membrane Integrity Assay from Promega

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CytoTox-ONE Homogeneous Membrane Integrity Assay from Promega

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