ApeKI from New England Biolabs

Product Specs
ItemApeKI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0643L
Quantity1250 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) White Lupin Adaptation to Moderately Calcareous Soils: Phenotypic Variation and Genome-Enabled PredictionPlants (Basel)March 2, 2023Paolo Annicchiarico, Abco J. de Buck, Dimitrios N. Vlachostergios, Dennis Heupink, Avraam Koskosidis, Nelson Nazzicari, Margherita Crosta3.6 ng of total adapters, and restricted the genomic DNA with ApeKI enzyme (NEB New England Biolabs, R0643L, Ipswich, MA, USA); then, the library was amplified with Kapa Taq polymerase Alpha (KAPA Library Amplification

(2) White Lupin Drought Tolerance: Genetic Variation, Trait Genetic Architecture, and Genome-Enabled PredictionInternational Journal of Molecular SciencesJanuary 25, 2023Luciano Pecetti, Paolo Annicchiarico, Margherita Crosta, Tommaso Notario, Barbara Ferrari, Nelson Nazzicari3.6 ng of total adapters and restricted the genomic DNA with ApeKI enzyme (NEB New England Biolabs, R0643L); then, the library was amplified with Kapa Taq polymerase Alpha (KAPA Library Amplification Readymix,

(3) Evolution of selfing syndrome and its influence on genetic diversity and inbreeding: A rangeâ€wide study in Oenothera primiverisAmerican Journal of BotanyMay 21, 2022Anita Cisternasâ€Fuentes, Tania Jogesh, Geoffrey T. Broadhead, Robert A. Raguso, Krissa A. Skogen, Jeremie B. Fantharringtonii W.L. Wagner, Stockh & W.M. Klein). For the digestion, the genome was cut with ApeKI (no. R0643; New England Biolabs, Ipswich, Massachusetts, USA) and then ligated with oligonucleotides, which included

(4) Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-RegimesFrontiers in Plant ScienceApril 21, 2021Srikanth Bollam, Kirandeep Kaur Romana, Laavanya Rayaprolu, Anilkumar Vemula, Roma Rani Das, Abhishek Rathore, Prasad Gandham, Girish Chander, Santosh P. Deshpande, Rajeev GuptaGenotyping was performed by following the GBS approach (Elshire et al., 2011), restriction enzyme ApeKI (NEB R0643L) used for complexity reduction. The GBS library was sequenced on IlluminaHiSeq 2500 (Illumina Inc.,

(5) A Genome-Wide Association Study Identifies Quantitative Trait Loci Affecting Hematological Traits in Camelus bactrianusAnimals (Basel)January 7, 2020Fucheng Guo, Liang Ming, Rendalai Si, Li Yi, Jing He, Rimutu Ji(GBS) [20,21]. The DNA was first digested using the methylation-sensitive restriction enzyme ApeKI (R0643L, New England Biolabs, Ipswich, MA, USA), and then the restriction fragments were ligated to a unique

(6) Pea genomic selection for Italian environmentsBMC GenomicsDecember 1, 2019Paolo Annicchiarico, Nelson Nazzicari, Luciano Pecetti, Massimo Romani, Luigi RussiQuant-iT™ PicoGreen® dsDNA assay kit (Life Technologies, P7589), each DNA sample (100 ng) was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter plus a common adapter. Equal volume of the ligated product

(7) Population Connectivity and Traces of Mitochondrial Introgression in New Zealand Black-Billed Gulls (Larus bulleri)Genes (Basel)November 9, 2018Claudia Mischler, Andrew Veale, Tracey van Stijn, Rudiger Brauning, John C. McEwan, Richard Maloney, Bruce C. Robertsonrestriction enzyme to use for digesting genomic DNA. For black-billed gulls, the restriction enzyme ApeKI (R0643L, New England Biolabs, Ipswich, MA, USA) was selected, and sample-specific barcode adapters were ligated

(8) Genomic Signatures of Adaptation to a Precipitation Gradient in Nigerian SorghumG3 Genes|Genomes|GeneticsOctober 1, 2018Marcus O. Olatoye, Zhenbin Hu, Fanna Maina, Geoffrey P. MorrisMorris et al. 2013). Briefly, individual DNA samples were digested using ApeKI restriction enzyme (NEB R0643L) followed by ligation of barcode and common adapters ligation using T4 DNA ligase (NEB M0202L). Ligated

(9) Population genomics of sorghum (Sorghum bicolor) across diverse agroclimatic zones of NigerGenomeApril 1, 2018Fanna Maina, Sophie Bouchet, Sandeep R. Marla, Zhenbin Hu, Jianan Wang, Aissata Mamadou, Magagi Abdou, Abdoul-Aziz Saïdou, Geoffrey P. Morrishighly differentiated genome regions (top 0.1% of SNPs). The dashed vertical lines represent a priori candidate genes. R0643L) and ligated with the barcode and common adapters using T4 DNA ligase (NEB M0202L). Ligated libraries

(10) Selection Mapping Identifies Loci Underpinning Autumn Dormancy in Alfalfa (Medicago sativa)G3 Genes|Genomes|GeneticsFebruary 1, 2018Gitanshu Munjal, Jingjie Hao, Larry R. Teuber, E. Charles Brummerpreviously in Li et al. (2014). Briefly, 100 ng of DNA from each sample was digested with ApeKI (NEB, R0643L). Two different types of adapters, a barcode adapter and a common adapter, were ligated with the DNA

(11) Genotyping-by-Sequencing and Its Exploitation for Forage and Cool-Season Grain Legume BreedingFrontiers in Plant ScienceMay 9, 2017Paolo Annicchiarico, Nelson Nazzicari, Yanling Wei, Luciano Pecetti, Edward C. Brummerwith a Quant-iT PicoGreen dsDNA assay kit, Life Technologies, P7589) was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter and a common adapter (7.0 ng of the adapter stock were

(12) A Parthenogenesis Gene Candidate and Evidence for Segmental Allopolyploidy in Apomictic Brachiaria decumbensGeneticsJuly 1, 2016Margaret Worthington, Christopher Heffelfinger, Diana Bernal, Constanza Quintero, Yeny Patricia Zapata, Juan Guillermo Perez, Jose De Vega, John Miles, Stephen Dellaporta, Joe Tohmeal. (2014) in their study of tetraploid alfalfa using the Elshire et al. (2011) protocol with ApeKI (R0643L; NEB). Over twice as many polymorphic SNPs were identiﬁed in that study; however, the mean percentage

(13) Mitochondrial Involvement in Vertebrate Speciation? The Case of Mito-nuclear Genetic Divergence in ChameleonsGenome Biology and EvolutionDecember 1, 2015Dan Bar-Yaacov, Zena Hadjivasiliou, Liron Levin, Gilad Barshad, Raz Zarivach, Amos Bouskila, Dan Mishmar(New England BioLabs #R0140S); ETFA—HindIII (Thermo #ER0501); and LYRM4—ApekI (New England BioLabs #R0643S). Assessing Possible Linkage between the Six nDNA Genes Which Correlated to the mtDNA Pattern It is

(14) Accuracy of genomic selection for alfalfa biomass yield in different reference populationsBMC GenomicsDecember 1, 2015Paolo Annicchiarico, Nelson Nazzicari, Xuehui Li, Yanling Wei, Luciano Pecetti, E. Charles Brummerthe protocol by [19] with modifications. Briefly, 100 ng of each DNA was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter and a common adapter. Equal volume of the ligated product

(15) In silico analysis identifies novel restriction enzyme combinations that expand reduced representation bisulfite sequencing CpG coverageBMC Research NotesDecember 1, 2014Daniel B Martinez-Arguelles, Sunghoon Lee, Vassilios Papadopoulosnumber AluI,AG|CT,37,CutSmart,80,No,R0137S BfaI,C|TAG,37,CutSmart,80,No,R0568S HaeIII,GG|CC,37,CutSmart,80,No,R0108S HpyCH4V,TG|CA,37,CutSmart,65,No,R0620S MluCI,|AATT,37,CutSmart,No,No,R0538S MseI,T|TAA,37,CutSmart,65,No,R0525S MspI,C|CGG,37,CutSmart,No,No,R0106S TaqI,T|CGA,65,CutSmart,80,dam,R0149S CviQI,G|TAC,25,NEB 3.1,No,No,R0639S CviAII,C|ATG,25,CutSmart,65,No,R0640S ApeKI,G|CWGC,75,NEB 3.1,No,Yes,R0643S Properties of the identified enzymes useful in RRBS

(16) A Saturated Genetic Linkage Map of Autotetraploid Alfalfa (Medicago sativa L.) Developed Using Genotyping-by-Sequencing Is Highly Syntenous with the Medicago truncatula GenomeG3 Genes|Genomes|GeneticsOctober 1, 2014Xuehui Li, Yanling Wei, Ananta Acharya, Qingzhen Jiang, Junmei Kang, E. Charles BrummerElshire et al. (2011) with minor modifications. In summary, 100 ng of each DNA was digested with ApeKI (R0643L; NEB), and then ligated to a unique barcoded adapter and a common adapter. Equal volumes of the ligated

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ApeKI

ApeKI from New England Biolabs

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New England Biolabs

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ApeKI from New England Biolabs

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