Fig 1: Protein and transcript accumulation in Arabidopsis Col-0 and mutant cotyledons. (a) Immuno-blot analyses of total protein extracts from cotyledons of Col-0, gun1-102, ftsh5-3, gun1-102 ftsh5-3, pub4-2 and gun1-102 ftsh5-3 pub4-7 grown on soil at 6 DAS. PVDF filters bearing fractionated total proteins were incubated with antibodies raised against LHCB5 antenna protein, VIPP1 plastid membrane chaperone, CLPB3 plastid unfoldase, cpHSC70-1 plastid chaperone, CPN60 plastid chaperonin, HSP90-1 cytosolic chaperone, HSC70-4 cytosolic chaperone and UBQ11 ubiquitin protein. Coomassie Brilliant Blue (CBB) stained SDS-PAGE is included as loading control. (b) Immunoblot analyses of chloroplast protein extracts from Col-0, gun1-102, ftsh5-3, gun1-102 ftsh5-3, pub4-2 and gun1-102 ftsh5-3 pub4-7 grown on soil at 6 DAS. PVDF filters bearing fractionated proteins were incubated with antibodies raised against UBQ11 ubiquitin protein. CBB-stained SDS-PAGE is included as loading control. (c) Relative expression level of HSFA2 gene determined by qRT-PCR analyses of total RNA extracted from cotyledons of Col-0, gun1-102, ftsh5-3, gun1-102 ftsh5-3, pub4-2 and gun1-102 ftsh5-3 pub4-7 grown on soil at 6 DAS. Error bars indicate standard deviations of three replicates. Asterisks indicate statistical significance with respect to Col-0 (black) or gun1-102 ftsh5-3 (red) as evaluated by Student’s t-test and Welch correction (* p < 0.05; *** p < 0.001; ns: not significant).