Fig 1: Effect of TQ on the activation status of JAK/STAT signaling in MV4-11 cells. The cells were incubated for 48 h with 5.5 µM TQ. The protein levels were determined by Jess simple western analysis. (A) Images of Jess simple western analysis for the target proteins before and after TQ treatment. (B) The bar graph demonstrates the reduction of STAT3, p-STAT3, STAT5, p-STAT5, JAK2, and p-JAK2 protein levels in MV4-11 cells after TQ treatment. Wilcoxon signed rank test was conducted, and the results are presented as medians and interquartile range (error bars) (n = 3), where * p < 0.05, ** p < 0.01, *** p < 0.001 are significant versus untreated control cells.