Description
Recombinant REAfinity™ Antibodies for reproducible flow cytometry results Recombinant antibodies are the next generation of engineered flow cytometry antibodies, and represent the first truly fundamental shift in antibodies since monoclonals were first developed 40 years ago. Recombinant antibodies are derived from a defined set of genes, and the production process is highly standardized. Therefore, these antibodies are consistent in their structure and performance, leading to high experimental reproducibility. Reproducibility not only ensures consistent results, it also helps you save time and goes easy on your budget.
REAfinity™ Antibodies are recombinant antibodies developed by Miltenyi Biotec that allow reproducible flow analysis. REAfinity Antibodies are expressed in mammalian cells under standardized in vitro culture conditions and highly controlled manufacturing practices that ensure long-term consistency across all lots. To ensure homogeneity within and among lots, each REAfinity Antibody is generated from a carefully defined sequence encoding a single heavy chain and a single light chain. Consequently, all produced antibody molecules consist of only those two immunoglobulin chains, eliminating the structural heterogeneity that can lead to variable specificity in traditional monoclonal antibodies. Furthermore, all REAfinity Antibodies include the same engineered human IgG1 constant region allowing the use of one isotype control for all REAfinity Antibodies.
In summary, the following features of REAfinity Antibodies make them the next generation antibodies for flow cytometry analysis:
- Generated in standardized culture conditions in mammalian cells
- Highly pure and free from hybridoma derived contaminating immunoglobulin impurities
- Engineered to lack binding to Fcγ receptors
- Recombinant antibodies with consistent human IgG1 isotype across the portfolio
- Validated for flow cytometry
- Conjugated to exclusive Vio® Dye fluorochromes for flexible multicolor flow cytometry
REAfinity Antibodies recognize human, mouse and rat antigens and are available against:
- Common immune antigens such as CD3, CD4, CD56
- Chemokine receptors
- Cell signaling proteins
- Stem cells, tumor cells and other cell types