Fig 2: B7-H3 protein expression in normal tissue, and in the primary and metastatic forms of osteosarcoma. B7-H3 expression was validated by western blot analysis: Lane 1, B7-H3 protein expression in normal tissue; lane 2, B7-H3 protein expression in the primary form of osteosarcoma; and lane 3, B7-H3 protein expression in the metastatic form of osteosarcoma. For control purposes, glyceraldehyde 3-phosphate dehydrogenase was used as a loading control.