Flow cytometry of LAMP1 in Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line and 3 µM monensin for 5 hours or (B) untreated. Samples were incubated in LAMP1 monoclonal antibody (Product # MA5-23578) followed by Mouse Anti-Human NCAM‚1/CD56 APC‚conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
Flow cytometric analysis of Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line and 3 uM monensin for 5 hours or (B) untreated were stained with mouse Anti-human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Product # MA5-23578) and mouse Anti-human NCAM-1/CD56 APC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.
Supplier Page from Thermo Fisher Scientific for LAMP1 Antibody PE