Fig 1: Expression of EAD‐pathway enzymes on PMNs is altered with age. (a) Bone marrow cells were isolated from young and old C57BL/6 mice, and basal expression of EAD‐pathway components was assessed by flow cytometry. (b) Blood was collected from young and old C57BL/6 mice and expression of EAD‐pathway components was assessed by flow cytometry. (a, b) We gated on PMNs (Ly6G+CD11b+ cells) and measured the expression (mean fluorescent intensity or MFI) of CD73, CD39, ADA, A1R, A2AR, A2BR and A3R. (a) Data shown are pooled from three separate experiments (n = 3 mice per age group) with each condition tested in triplicate. (b) Data are pooled from four separate experiments with 7 mice per age group. *Significant differences from uninfected controls calculated by Student's t‐test