Fig 2: LM11A-31 pretreatment inhibits p75NTR and inflammation-related transcription factors expression at 24 h after PM induction. Western blot analysis of p75NTR, p-NF-κBp65/NF-κBp65 and C/EBPβ protein levels in the cortex a, c and hippocampus b, d from rats pretreated with vehicle or LM11A-31 at 24 h after PM induction. Proteins were normalized to β-actin. Data are presented as the mean ± SEM (n = 5 rats). *p < 0.05, **p < 0.01, ***p < 0.001

Fig 3: Nerves in the tumor microenvironment of lung cancer do not express NGF, proNGF, TrkA, p75NTR and sortilin. (A) Immunohistochemical detection of the pan-neuronal marker PGP9.5 was used to detect nerves in lung cancers. The expression of NGF (B), proNGF (C), TrkA (D), p75NTR (E) and sortilin (F) was not detected in serial sections. Black arrows indicate a nerve trunk composed of many axons. Scale = 25 μm.

Fig 4: The expression of proBDNF/p75NTR and p75NTR cellular distribution at 24 h after PM induction. a, b Western blot analysis of proBDNF/p75NTR protein levels in the cortex and hippocampus at 24 h post-infection. Proteins were normalized to β-actin. c Representative immunofluorescence colocalization images of p75NTR/NeuN, p75NTR/Iba1, p75NTR/GFAP in the cortex at 24 h post-infection. Neuron cells, microglial cells, and astrocytes are labeled with NeuN (green), Iba-1 (green), and GFAP (green), respectively. (p75NTR = red and DAPI = blue). Data are presented as the mean ± SEM (n = 2–6 rats). **p < 0.01. Scale bar = 20 μm

Fig 5: p75NTR expression in cervical cancers and normal cervical tissue. Immunohistochemical detection of p75NTR in normal cervical tissues (A), adenocarcinomas (AC) (B) and squamous cell carcinomas (SCC) (C, D). Scale bars: 50 μm (A‐D). (E) Digital quantification of p75NTR expression was performed and h‐scores were calculated. Data are expressed as medians (horizontal line in the center of the box) and box limits indicate the interquartile range (IQR) with the whiskers extending 1.5 times the IQR from the 25th and 75th percentiles; outliers are represented by dots. *P < .005 compared to normal tissue expression, assessed using the Kruskal‐Wallis test with adjustment for multiple comparisons