Fig 2: Renal microscopic IHC images. (a, d) IHC of Ly6G and Nlrp3 in the kidney after CM injection. The upper row of this group of pictures shows the renal cortex (CO) and the second row the renal medulla (ME). From left to right, each one of the four pictures indicates 1 h, 24 h, 48 h, and 72 h after CM injection. (b, e) Ly6G and Nlrp3 without primary antibody in the kidney as negative control kid (-). (c, f) Ly6G and Nlrp3 in the spleen as positive control Spl (+). (g, h) Are the H-score for IHC of Ly6G and Nlrp3; ∗P < 0.05, ∗∗P < 0.005, and ∗∗∗P < 0.0001. Original magnification: 400x.