Fig 1: Candidate verification by RNA in situ or western blot. (A–E) RNAscope in situ hybridization was performed on coronal sections of wild-type mouse brains in the mPFC and NAc with a Prkcg probe. Prkcg mRNA was expressed in both the mPFC and the NAc. Scale bars are 200 μm. B, bregma; cg1, cingulate cortex, area 1; PrL, prelimbic cortex; IL, infralimbic cortex; aca, anterior commissure, anterior part; AcbC, accumbens nucleus, core; LAcbSh, lateral accumbens shell; AcbSh, accumbens nucleus, shell; DEn, dorsal endopiriform claustrum; IEn, intermediate endopiriform claustrum; VP, ventral pallidum. (F–G) Western blot analysis for the phosphorylation of Csnk1a1 at T321. All data are shown as mean ±SEM. *P < 0.05, one-way ANOVA tests for CUMS + saline compared with Control + saline; **P < 0.01, one-way ANOVA tests for CUMS + saline compared with CUMS + ketamine.