Fig 1: PD-L1, ICOSLG, and B7-H3 are the direct target genes of miR-326.A Software (miRBase, PicTar, and TargetScan) prediction of the miR-326-targeted genes. B–D 293T cells were transfected with luciferase reporter plasmid containing PD-L1/B7-H3/ICOSLG wild-type or mutant-type 3' UTR and miR-NC (n = 3). E Schematic diagram of the binding sites between miR-326 and the target genes (PD-L1, B7-H3, and ICOSLG) and their mutant sequence. All data were presented as mean ± SEM. Comparisons between groups for statistical significance were performed with two-way ANOVA with Tukey’s post hoc test (B–D). ***P < 0.001 versus 3' UTR + miR-NC. ###P < 0.001 versus 3' UTR + miR-326.