Anti-Puromycin [3RH11] Antibody, 100ug EQ0001 from Kerafast

Product Specs
ItemAnti-Puromycin [3RH11] Antibody, 100ug
CompanyKerafast
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberEQ0001
Quantity100ug
ApplicationsWestern blotting, ELISA and Immunofluorescence microscopy
FormatLiquid
PurityProtein G
TypeAntibody
TargetPuromycin
Clone3RH11
Immunogenpuromycin hydrochloride
HostMouse
ClonalityMonoclonal
IsotypeIgG1

Add to Compare List

Kerafast

2 Shaker Road, Unit B101

Shirley, MA 01464

United States

Phone: 1-800-546-1760
1-617-336-3228 (Mon–Fri 8:30AM–5:30PM EST)
Fax: 1-866-727-9826

Email: CustomerService@kerafast.com

Company Profile

Website: www.kerafast.com

(1) Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processingRNA BiologyDecember 31, 2023Mason A. McCool, Carson J. Bryant, Hannah Huang, Lisa M. Ogawa, Katherine I. Farley-Barnes, Samuel B. Sondalle, Laura Abriola, Yulia V. Surovtseva, Susan J. Basergablotting. The following primary antibodies were used: α-p53 (Santa Cruz, sc-126), α-puromycin (Kerafast, EQ0001), α-HA (clone 12CA5), and α-β-actin (Sigma Aldrich, A1978), α-POLR1A (Santa Cruz, sc-48,385), α-FBL

(2) microRNAs slow translating ribosomes to prevent protein misfolding in eukaryotesThe EMBO JournalSeptember 18, 2023Hiroaki Sako, Mohieldin Youssef, Olga Elisseeva, Takayuki Akimoto, Katsuhiko Suzuki, Takashi Ushida, Tadashi YamamotoMillipore), anti‐AGO2 (2897S, Cell Signaling), anti‐DROSHA (sc‐393591, Santa Cruz), anti‐puromycin (EQ0001, Kerafast), anti‐TUBG1 (T3320, Sigma), anti‐GAPDH (2118S, Cell Signaling), anti‐rabbit IgG (NA9340,

(3) AGAP1-associated endolysosomal trafficking abnormalities link gene–environment interactions in neurodevelopmental disordersDisease Models & MechanismsSeptember 1, 2023Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruer(1:500; Abcam 4837). Newly synthesized proteins were labeled with mouse anti-puromycin (1:1000; Kerafast, EQ0001) and their levels normalized using Ponceau staining. The following secondary antibodies were used: goat

(4) The translation initiating factor eIF4E and arginine methylation underlie G3BP1 function in dendritic spine development of neuronsThe Journal of biological chemistryAugust 1, 2023Dong R, Li X, Flores AD, Lai KO.BSA in D-PBS) for 30 min. After blocking, the cells were incubated with puromycin antibody (Kerafast #EQ0001, 1:500 diluted in blocking buffer) overnight at 4 °C and subjected to immunofluorescence staining on

(5) Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunctionFrontiers in Molecular BiosciencesJune 22, 2023Joseph P. Clarke, Patricia A. Thibault, Sakina Fatima, Hannah E. Salapa, Subha Kalyaanamoorthy, Aravindhan Ganesan, Michael C. Levinanti-hnRNPA1 (4B10),Millipore Sigma,Cat# 05-1521 Mouse monoclonal anti-Puromycin [3RH11],Kerafast,Cat# EQ0001 Alexa Fluor 488 Donkey anti-mouse IgG (H+L),Jackson ImmunoResearch,Cat# 715-546-151 Alexa Fluor 488

(6) NAD(+) repletion with niacin counteracts cancer cachexiaNature communicationsApril 3, 2023Beltrà M, Pöllänen N, Fornelli C, Tonttila K, Hsu MY, Zampieri S, Moletta L, Corrà S, Porporato PE, Kivelä R, et al.(1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614,

(7) AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorderbioRxivFebruary 4, 2023Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruerα eIF2S1 (Abcam 4837). Newly synthesized proteins labeled with 1:1000 mouse α puromycin (Kerafast, EQ0001) and normalized to Ponceau staining. Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL

(8) Pharmacological vitamin C inhibits mTOR signaling and tumor growth by degrading Rictor and inducing HMOX1 expressionPLoS geneticsFebruary 1, 2023Qin S, Wang G, Chen L, Geng H, Zheng Y, Xia C, Wu S, Yao J, Deng L.Cell Signaling Technology), p4EBP1 (1:1000; 9451S; Cell Signaling Technology), anti-Puromycin (1:1000, EQ0001, Kerafast), SVCT1 (1:1000; NBP2-13318; Novus), SVCT2 (1:1000; NBP2-13319; Novus), AMPK (1:1000; 2532S;

(9) Real-time imaging of RNA polymerase I activity in living human cellsThe Journal of cell biologyJanuary 2, 2023Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, et al.to polyvinylidene difluoride membranes followed by incubation with anti-puromycin antibody (Kerafast-EQ0001, 1 µg/ml in Tris-buffered saline containing Tween-20) overnight at 4°C, goat anti-mouse HRP secondary

(10) Intensive Care Unit Acquired Weakness Is Associated with Rapid Changes to Skeletal Muscle ProteostasisCellsDecember 11, 2022Mustafa Ozdemir, Matthew P. Bomkamp, Hayden W. Hyatt, Ashley J. Smuder, Scott K. Powerssoftware (Li-Cor Biosciences, Lincoln, NE, USA). Primary antibodies of interest were anti-puromycin (#EQ0001) (Kerafast, Boston, MA, USA), anti-mTOR (#2983), anti-p-mTOR (#5536), anti-AKT (#2938), anti-p-AKT (#9271),

(11) Human pre-60S assembly factors link rRNA transcription to pre-rRNA processingRNA (New York, N.Y.)November 2, 2022McCool MA, Buhagiar AF, Bryant CJ, Ogawa LM, Abriola L, Surovtseva YV, Baserga SJ.Santa Cruz sc-126), α-p21 (dilution 1:400, Santa Cruz sc-6246), α-puromycin (dilution 1:10,000; Kerafast EQ0001), and α-β-actin (dilution 1:30,000; Sigma Aldrich A1978). The western blots were developed with enhanced

(12) Ribosome changes reprogram translation for chemosurvival in G0 leukemic cellsScience advancesOctober 28, 2022Datta C, Truesdell SS, Wu KQ, Bukhari SIA, Ngue H, Buchanan B, Le Tonqueze O, Lee S, Kollu S, Granovetter MA, et al.(ab134053), and ATF4 (ab23760) (used for Western blot) were from Abcam; and anti-puromycin [3RH11] (EQ0001) was from Kerafast. Mass spectrometry Multiplex quantitative proteomics analysis (TMT spectrometry)

(13) miR-124-dependent tagging of synapses by synaptopodin enables input-specific homeostatic plasticityThe EMBO journalOctober 17, 2022Dubes S, Soula A, Benquet S, Tessier B, Poujol C, Favereaux A, Thoumine O, Letellier M.modifications (Merck). Labeling of newly synthesized SP was performed using mouse anti‐puromycin antibody (EQ0001, Kerafast, 1:2,500) in combination with rabbit anti‐SP antibody (163002, Synaptic System, 1:600) and

(14) Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myelomaScience advancesSeptember 30, 2022Sharma A, Nair R, Achreja A, Mittal A, Gupta P, Balakrishnan K, Edgar CL, Animasahun O, Dwivedi B, Barwick BG, et al.were obtained from Sigma-Aldrich. REDD1 (#10638-1-AP) was obtained from Proteintech. Anti-puromycin (#EQ0001) was obtained from Kerafast. Bioinformatics analysis Unpaired analysis GSEA was performed on DESeq2-normalized

(15) Mettl3-dependent m⁶A modification attenuates the brain stress response in DrosophilaNature communicationsSeptember 14, 2022Perlegos AE, Shields EJ, Shen H, Liu KF, Bonini NM.10/10/19–53 ug/ml), anti-drpr (1:400, DSHB-5D14, 6/22/17−36 ug/ul), anti-puromycin (1:1,000, Kerafast, EQ0001, 200517), anti-hsf (1:20:000, anti-rabbit, gift from John Lis60,61). Rabbit anti-Ythdc1 (1:5000), affinity

(16) 4-Hydroxyisoleucine inhibits tumor growth by triggering endoplasmic reticulum stress and autophagyCurrent Research in Pharmacology and Drug DiscoverySeptember 9, 2022Peng Li, Yonghui Yang, Lu Qiu, Guangming Wan, Baomei Yuan, Yahong Wu, Yanfeng Gao, Guodong LiPERK (Abcam,ab229912,UK), p-PERK(Affinity, DF-7576), LC3B (Abcam, ab192890, UK), Puromycin (Kerafast, EQ0001,USA). The band density was measured (Quantity One software) and normalized to β-Actin. 2.7Transmission electron microscopy

(17) White spot syndrome virus directly activates mTORC1 signaling to facilitate its replication via polymeric immunoglobulin receptor-mediated infection in shrimpPLOS PathogensSeptember 6, 2022Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang(p)-S6K1 (Thr389) (Catalog No. AP0564, ABclonal, Wuhan, China, 1:500); anti-puromycin (Catalog No. EQ0001, Kerafast, 1:1000), and gently shaken overnight at 4°C. After washing three times with TBST (0.1% Tween-20

(18) L-Arginine/nitric oxide regulates skeletal muscle development via muscle fibre-specific nitric oxide/mTOR pathway in chickensAnimal NutritionSeptember 1, 2022Ruxia Wang, Kelin Li, Li Sun, Hongchao Jiao, Yunlei Zhou, Haifang Li, Xiaojuan Wang, Jingpeng Zhao, Hai LinBoston, USA), anti-p70S6K (#2708, anti-rabbit, Cell Signaling Technology, Boston, USA), anti-puromycin (EQ0001, anti-mouse, Kerafast, Boston, USA), and anti-tubulin (AT819, anti-mouse, Beyotime, Nanjing, China)

(19) Serotonergic neuron ribosomal proteins regulate the neuroendocrine control of Drosophila developmentPLOS GeneticsSeptember 1, 2022Lisa Patricia Deliu, Michael Turingan, Deeshpaul Jadir, Byoungchun Lee, Abhishek Ghosh, Savraj Singh GrewalPrimary antibodies used were anti-puromycin (3RH11) antibody (1:1000, Kerafast, Boston, USA, cat. no. EQ0001), anti-eIF2alpha (1:1000, AbCam #26197). Secondary antibodies were purchased from Santa Cruz 144 Biotechnology

(20) An aqueous extract of the brown alga Eisenia bicyclis extends lifespan in a sex-specific manner by interfering with the Tor-FoxO axisAging (Albany NY)August 31, 2022Navid Tahanzadeh, Mirjam Knop, Yvonne Seidler, Sebastian Dirndorfer, Kai Lürsen, Iris Bruchhaus, Roman Lang, Gerald Rimbach, Thomas Roederfor 1.30 h. The membrane was incubated with anti-puromycin (3RH11) antibody (Kerafast, Boston, USA, EQ0001) over night at 4° C. After 3 times washing with TBST, HRP-coupled anti-rabbit secondary antibody was

Write a Review

(1)

6/13/2022Good Puromycin Antibody for Western Blot Chang Seok LeeA mouse model with I4895T mutation in type 1 ryanodine receptor displays myopathy including muscle weakness and atrophy. In vivo protein synthesis with puromycin labeling showed decrease of newly ... Read Review

Anti-Puromycin [3RH11] Antibody, 100ug from Kerafast

Kerafast

Citations (145)

Reviews

Anti-Puromycin [3RH11] Antibody, 100ug from Kerafast

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map